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Inflammatory Cytokines Regulate Glycoprotein Subunit β5 of Thyrostimulin through Nuclear Factor-B

Department of Endocrinology and Diabetes (C.S., Y.Ok., H.S., N.O., H.A., Y.Oi.), Nagoya University Graduate School of Medicine and Department of Metabolic Medicine (H.N.), Nagoya University, Aichi 466-8550, Japan; and Department of Endocrinology (Y.I.), Kochi Medical School, Koci 781-51, Japan

Address all correspondence and requests for reprints to: Hiroshi Nagasaki, Department of Metabolic Medicine, Nagoya University, 65 Tsurumai-cho, Naka-ku, Nagoya City, Aichi 466-8550, Japan. E-mail: nagasaki{at}med.nagoya-u.ac.jp.

Thyrostimulin is a heterodimeric hormone comprised of two glycoprotein hormone subunits, namely glycoprotein hormone subunit 2 and glycoprotein hormone subunit β5 (GPB5). Immunological studies have revealed that both subunits colocalize in human pituitary corticotroph cells. Although recombinant thyrostimulin protein selectively activates the TSH receptor and has thyrotropic activity in rats, its biological functions have not been clarified. To explore the physiological regulators for the GPB5, the 5'-flanking region of the GPB5 coding sequence up to 3-kb upstream was analyzed by luciferase reporter assays. We found that nuclear factor-B (NF-B) markedly activated GPB5 transcription. Disruption of the putative NF-B-binding motifs in the GPB5 5'-flanking region silenced the GPB5 activation by p65. Chromatin immunoprecipitation assays revealed that recombinant p65 bound to the predicted NF-B-binding sites. Because NF-B is known to associate with acute phase inflammatory cytokines, we examined whether TNF or IL-1β could regulate GPB5. Both these cytokines activated GPB5 transcription by 2- to 3-fold, and their effects were abolished by the addition of MG132, a NF-B inhibitor. Our results suggest that inflammatory cytokines positively regulate thyrostimulin through NF-B activation.