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DETAILED DESCRIPTION OF TECHNIQUE FOR ANDROGEN ASSAY BY THE CHICK COMB METHOD¹

the Laboratories of The Mount Sinai Hospital NEW YORK CITY

Abstract

CLINICAL INVESTIGATIONS involving assays for androgenic activity have lagged behind those related to the estrogens because of the lack of a convenient and reliable bio-assay technique. Of all of the numerous methods which have been proposed from time to time, that of Gallagher and Koch (1), based on the capon’s comb as a quantitative indicator of the potency of injected androgenic material, has been used most extensively. During the past four years we have been engaged in developing a bio-assay method for androgenic activity in urine and tumors, which is relatively simple and of recognizable reliability (for preliminary reports on this work see 2, 3, 4). This investigation has finally been concluded and a detailed report on the work is now nearing completion. Along with a careful description of the proced-ure, this report will include the evidence, both historical and experimental, which guided us in the development of our method; it will contain also a minute statistical analysis of the data, collected over more than a year on about 1500 chicks, which led us to the final dose-response equation.

Footnotes

¹ This investigation was supported in part by grants from the Friedsam Foundation.

Received April 15, 1942.