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Endocrinology, doi:10.1210/endo-49-5-617
Endocrinology Vol. 49, No. 5 617-623
Copyright © 1951 by the Endocrine Society.
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A DIFFUSION METHOD FOR THE ESTIMATION OF FORMALDEHYDOGENIC STEROID*

VINCENT P. HOLLANDER**, SANDINA DIMAURO and OLOF H. PEARSON***

From the Division of Clinical Investigation, Sloan-Kettering Institute, Memorial Center for Cancer and Allied Diseases New York

Abstract

ONE method for the estimation of cortical hormone in urine is the determination of the formaldehyde produced by the oxidation of the ketol side chain with periodic acid (Lowenstein, Corcoran and Page, 1946). In order to minimize the contribution of non-specific organic material, Daughaday, Jaffee and Williams (1948) developed a method in which the formaldehyde is distilled before it is estimated by means of the color reaction with chromotropic acid. Corcoran and Page (1948) have developed similar procedures.

It occurred to us that isothermal distillation of the formaldehyde by means of Conway cells (Conway, 1947) would be useful in this determination. One such method has been reported by Bassil and Hain (1950) but is inconvenient since it requires three to four days to reach equilibrium and does not permit quantitative recovery. The solution of this problem is to allow the distillation to occur directly into the chromotropic acid reagent, which is dissolved in 15 M

Footnotes

* This study was supported by grants from the Damon Runyon Memorial Fund for Cancer Research, the American Cancer Society, the National Cancer Institute of the National Institutes of Health, Public Health Service, and the Atomic Energy Commission Contract No. AT(30-l)-910.

** Damon Runyon Clinical Research Fellow.

*** Damon Runyon Senior Clinical Research Fellow.

Received July 16, 1951.






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Copyright © 1951 by The Endocrine Society