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A SIMPLIFIED TEST FOR ANTI-DIABETIC ACTIVITY

Division of Biological Research, G. D. Searle & Co. Chicago, Illinois

Abstract

In A recent study of the quantitative relationships among glycosuria, food intake and body weight in alloxan-diabetic rats, an index of the severity of diabetes was established which was independent of changes in intake and body weight (Sturtevant, et al., 1954). This index was obtained by dividing the glucose excretion by the amount of food consumed. The present report describes the effect of a known anorexigenic drug and of several dose-levels of insulin on this index.

MATERIALS AND METHODS

Forty-two male Sprague-Dawley rats, which varied from 100 to 300 gm. in weight, were given two successive intraperitoneal injections of alloxan monohydrate, 160 mg. per kg., 48 hours apart. After the 3 to 4 weeks required for stabilization of the diabetic glycosuria (Sturtevant, et al., 1952), the rats were placed in individual metabolism cages and offered tap water and a medium carbohydrate diet (Table 1) ad libitum. The diet

Received October 7, 1953.