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Endocrinology, doi:10.1210/endo-74-3-314
Endocrinology Vol. 74, No. 3 314-324
Copyright © 1964 by the Endocrine Society.
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A Study of the Immunological and Biological Properties of a Follicle-Stimulating Hormone

CHARLES A. ELY and THOMAS TALLBERG1

Department of Anatomy, College of Physicians and Surgeons, Columbia University New York City

This investigation was supported in part by Grant C-5239-1-3 from the National Institutes of Health of the USPHS and Grant DRG-537 from the Damon Runyon Memorial Fund.

Abstract

Two male rabbits were immunized with NIH FSH and the immunological properties of the antiserum were studied by means of agar gel diffusion and agar and starch immunoelectrophoresis. The 2 antisera absorbed with a variety of sheep tissues including sheep LH revealed 2 antigenantibody systems when reacted against the hormone. Each system formed a characteristic precipitin band. One of the 2 bands was identical in both sera, but the second differed. On the other hand, the hemagglutination titers were the same for the 2 antisera. A variety of biological assays in normal and hypophysectomized rats revealed that antiserum from the 2 rabbits were different with respect to antihormone potency, as indicated by organ weights and the histological differences. With these facts in mind, it was possible to postulate which of the 2 precipitin bands was the likely site of anti-FSH activity. The anti- FSH sera clearly inhibited the effect of mouse pituitary saline extracts on the assay rat ovary. (Endocrinology 74: 314, 1964)

Footnotes

1 Research Associate in the Department of Anatomy, 1961–63.

Received April 17, 1963.






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Copyright © 1964 by The Endocrine Society