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Recovery and Partial Purification of FSH and LH During the Purification of TSH from Human Pituitary Glands¹

Department of Physiology and Department of Biochemistry, Division of Basic Health Sciences, Emory University Atlanta 22, Georgia
National Institute of Arthritis and Metabolic Diseases, National Institutes of Health Bethesda, Maryland

Abstract

Extracts of human pituitary glands containing FSH, LH and TSH were fractionated by chromatography on CM-C. FSH was not adsorbed on CM-C at pH 5.85 in 0.005M citrate-phosphate buffer and was thus separated from LH and TSH, which are strongly bound to CM-C under these conditions. Further purification of FSH was accomplished by gel filtration on G-100 and chromatography on DEAE-C. The final product had a potency 75 times NIH-FSH-SI/mg. The TSH and LH fractions, which were bound to CM-C, were eluted with a gradient, filtered on Sephadex G-100 and then chromatographed on DEAE-C in order to separate the 2 hormones. The TSH obtained had a potency of 20 IU/mg. The LH potency was 3 times NIHLH- SI/mg, according to the OAAD assay of Parlow. (Endocrinology 76: 27, 1965)

Footnotes

¹ This investigation was supported in part by USPHS Grants AM-06388-02 and AM-3598-04. Publication #649 of the Div. of Basic Health Sciences, Emory University, Atlanta, Ga.

² Emory University.

³ National Institute of Arthritis and Metabolic Diseases.

Received May 29, 1964.