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A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the in Vitro Study of Adrenal Metabolism

Department of Medicine, Vanderbilt University School of Medicine Nashville, Tennessee 37203

These studies were supported in part by the following grants-in-aid from the USPHS, NIH: 5-TOI-AM-05092, 2-FO5-TW-1135, 5-ROI-AM-05318, and 4-K6-AM-3782.

Abstract

A method of preparation of a rat adrenal cell suspension is described. The adrenal cell suspension is much more sensitive to the steroidogenic action of ACTH than are adrenal slices or homogenates. The adrenal cell suspension is also efficient in converting exogenous precursors into corticosterone. It is suggested that the adrenal cell suspension might prove to be a versatile tool in adrenal physiology. (Endocrinology 82: 1053, 1968)

Received September 11, 1967.

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