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Displacement of Estradiol-17β from the Uterus by Estradiol-17: Effect on Template Capacity of Uterine Chromatin

Departments of Obstetrics and Gynecology and of Biochemistry, University of Nebraska, College of Medicine Omaha, Nebraska 68105

Abstract

Administration of 6,7-³H-estradiol- 17β to mature ovariectomized rats results in the labeling of 2 pools of estradiol-17β in the uterus. Half-lives of the 2 pools are 0.7 and 5 hr. Intraluminal (IL) uterine application of 10 µg of estradiol-17 6 hr after administration of ³Hestradiol- 17β is capable of enhancing the rate of displacement of the previously bound active estrogen from the uterus. By the 12th hr the uterine levels are 30-50% less than in saline treated controls. The reduced estradiol-17β content is reflected in the relative labeling of uterine chromatin prepared from the 2 groups. When assayedassayed for its capacity to serve as a template for DNA-dependent RNA polymerase, it was found that treatment with estradiol-17 prevented the additional increase in template capacity of uterine chromatin normally observed to occur between the 6th and 12th hr after treatment with estradiol-17β. These results suggest that estradiol- 17 and perhaps other estrogenic compounds with subdued biological activity may well able to inhibit certain estrogen induced uterine responses which are in progress at the time treatment. (Endocrinology 83: 585, 1968)

Footnotes

Population Council and (HD-02851) from the USPHS.

¹ Career Development Awardee (HD-038656), National Institute of Child Health and Human Development.

² Predoctoral Fellow in the Reproductive Biology Training Program (USPHS, 2T1HD-18-06) of Department of Obstetrics and Gynecology, University of Nebraska College of Medicine.

Received December 15, 1967.

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