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Endocrinology, doi:10.1210/endo-87-6-1351
Endocrinology Vol. 87, No. 6 1351-1354
Copyright © 1970 by the Endocrine Society.
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Effect of L-Epinephrine and Triamcinolone on the Incorporation of Acetate-2-14C and 32Pi Into Phospholipids and Neutral Lipids in Liver Slices from Adrenalectomized Rats, in Vitro

KARL Y. HOSTETLER1 and ROBERT C. HAYNES, JR.2

Departments of Medicine and Pharmacology, Case Western Reserve University Cleveland, Ohio 44106

Abstract

In liver slices from adrenalectomized rats, L-epinephrine (3x10–6M) and triamcinolone (1x10–6M) stimulated gluconeogenesis from L-alanine, but neither treatment significantly changed the concentration of tissue neutral lipids or phospholipids. Phosphatidylinositol was not found in the lipid extracts of liver slices from rats 3–5 days after adrenalectomy. L-Epinephrine decreased the incorporation of acetate-2-14C into phosphatidylcholine, phosphatidylethanolamine and triglycerides, but did not affect incorporation into tissue free fatty acids, phosphatidylserine, cholesterol, or cholesterol esters. L-Epinephrine caused a 3.4-fold increase in 32Pi incorporation into phosphatidylserine, but caused much smaller per cent increases in the incorporation of 32Pi into phosphatidylcholine and phosphatidylethanolamine. Triamcinolone had no effect on the tissue levels or on the incorporation of acetate-2-14C into tissue neutral lipids and phospholipids. Incubation of liver slices in vitro for 3 hr decreased the concentration of cholesterol esters and phosphatidylcholine and raised the tissue free fatty acid concentration.

Footnotes

Supported by Grant A-1256, from the National Institute of Arthritis and Metabolic Diseases, USPHS.

1 USPHS Postdoctoral Fellow in Medicine, Department of Medicine, Case Western Reserve University, Grant AM-1005.

2 Present address: Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Va. 22903.

Received February 10, 1970.






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Copyright © 1970 by The Endocrine Society