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A Microgel Electrophoretic Analysis of the Colloid Proteins in Single Rat Thyroid Follicles. II. The Protein Concentration of the Colloid in Single Rat Thyroid Follicles

Department of Anatomy, University of Goteborg Sweden

Abstract

Samples of the colloid in rat thyroid follicles were obtained by micropuncture of superficial isthmic follicles in vivo and of superficial and deeper follicles in saline perfused thyroids. The protein compositions of the samples were analyzed by microgel electrophoresis. The amount of protein in the different fractions in the colloid samples was determined by microdensitometry. The sample volumes were estimated by measuring the amount of radioactivity in a solution occupying the same space in the tip of the collecting pipette as the colloid sample. Samples of colloid containing 19S thyroglobulin (TG) and proteins migrating more slowly than 19S TG had low protein concentrations, about 5 g/100 ml. Samples containing a prealbumin fraction in addition to the large proteins in the previous group had significantly higher protein concentrations, about IS g/100 ml. Colloid samples containing additional proteins (an albumin- like protein and a globulin) generally had mean protein concentrations around 10 g/100 ml. The mean protein concentration for the whole material was 11.0 g/100 ml (± 7.4 SD n = 40). (Endocrinology 91: 1300, 1972)

Received April 3, 1972.

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