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Endocrinology, doi:10.1210/endo-92-4-1153
Endocrinology Vol. 92, No. 4 1153-1160
Copyright © 1973 by the Endocrine Society.
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Ultrastructural and Light Microscopic Observations of Rat Pituitary LH-Containing Gonadotrophs Following Injection of Synthetic LH-RH1

D. MENDOZA2, A. ARIMURA and A. V. SCHALLY

Veterans Administration Hospital Minneapolis, Minnesota
Department of Medicine, Tidane University School of Medicine, Endocrine and Polypeptide Laboratories, Veterans Administration Hospital New Orleans, Louisiana 70146

Abstract

The effect of luteinizing hormonereleasing hormone (LH—RH) on pituitary cytology was investigated in intact, hemicastrated, and castrated male and female rats using electron and light microscopy. Two hundred ng of LH—RH were injected into the carotid artery, and the animals were sacrificed 1, 3, and 9 min after the injection. Study of LH—containing gonadotrophs by an immunocytochemical method using peroxidase- labeled antibody showed depletion of peroxidase reactivity of LH cells 1 min after injection of LH—RH in intact and hemicastrated proestrous rats, and after 9 min in the castrated females. Serum LH levels measured by radioimmunoassay were found to be significantly elevated 3 and 9 min after LH—RH injection in intact, hemicastrated and castrated male and female rats. Serum FSH levels had risen 1,3, and 9 min after injection of LH—RH in the intact males and hemicastrated proestrous females, had fallen 1 and 3 min after injection in the hemicastrated male and had not changed 1, 3, or 9 min after injection in the castrated male and females. (Endocrinology 92: 1153, 1973)

Footnotes

1 These studies were supported by PHS Grants AM-09094 and AM-07467 and Veterans Administration.

2 Present address: University Hospital, Cali, Colombia, S.A. Reprint requests to be sent to VA Hospital, New Orleans, La. 70146.

Received September 15, 1972.






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Copyright © 1973 by The Endocrine Society