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Cholesterol Sulfate: Some Aspects of Its Biosynthesis and Uptake by Tissues from Blood

RICHARD B. HOCHBERG, SHOSHANA LADANY and SEYMOUR LIEBERMAN

Departments of Biochemistry, of Obstetrics and Gynecology, and The International Institute for the Study of Human Reproduction, College of Physicians and Surgeons, Columbia University New York, New York 10032

This work was supported by Grants AM-00100, 5T1 HD-00013, 1P01 HD-OSO77 and Research Fellowship grant 5F2 AM-31,354 (To R.B.H.) from the National Institutes of Health of the United States Public Health Service.

Abstract

The ability of a variety of tissues of the guinea pig to synthesize cholesterol sulfate from ³⁵SO₄= has been measured. Liver, kidney, skin, and lungs were active whereas, surprisingly, synthesis of the conjugate in steroid-producing endocrine organs (adrenals, testes and ovaries) was barely detectable. Following its injection intravenously, the uptake of cholesterol sulfate by various tissues of the rat has been determined. The endocrine glands did not significantly remove this conjugate from the circulation. Other tissues that were studied varied not only in this regard but also in their ability to cleave the conjugate. An attempt to find a sterol sulfate precursor of cholesterol sulfate was made by incubating rat liver homogenates either with ³⁵S-sulfate or with ¹⁴C-mevalonic acid, but no labeled precursor was found. The origin of the cholesterol sulfate, found in abundance in adrenal tissue, still remains to be determined. (Endocrinology 94: 207, 1974)

Footnotes

Present-address: Department of Biodynamics, The Weizmann Institute of Science, Rehovot, Israel.

Received March 5, 1973.

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