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Purified Somatotrophs from Rat Adenohypophysis: Response to Secretagogues¹

Department of Physiology, Queen's University at Kingston Kingston, Ontario, Canada Department of Biology, The Pennsylvania State University University Park, Pennsylvania

Abstract

We previously reported methods for the preparation of enriched and purified dispersed somatotrophs from the rat adenohypophysis. We now demonstrate that the somatotrophs retain (a) their GH content during the purification proceduresand (b) their responsiveness to dibutyryl cyclic AMP and to a partially purified hypothalamic extract, rich in GHRF (GH-releasing factor) activity. This dispersed somatotroph preparation may provide a novel and useful tool in the study of the mechanisms governing the synthesis and release of GH. (Endocrinology 94: 1525, 1974)

Footnotes

¹ Supported by the Medical Research Council of Canada, the J. P. Bickell Foundation and the National Science Foundation GB-33686. W. C. Hymer is the recipient of PHS Research Career Development Award no. 1-K04-AM-15808.

Received July 11, 1973.