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Dynamics of Insulin Release and Microtubular-Microfilamentous System. VI. Effect of D₂O

Laboratory of Experimental Medicine, University of Brussels Brussels, Belgium Institute Histology, University of Geneva Geneva, Switzerland

Abstract

The present experiments were designed to assess the extent to which the β-cell microtu-bular-microfilamentous system contributes to the release of insulin evoked by glucose or sulfonylurea in the isolated perfused rat pancreas. Deuterium oxide (100%, v/v), which is thought to interfere with micro-tubular-microfilamentous function, was found to suppress insulin release during both the early and late phases of the secretory process. The inhibitory effect of D₂O was reversible. When the pancreases) were first exposed to cytochalasin B (10 µg/ml) and, thereafter, (to both cytochalasin B and D₂O, an escape)phenomenon from the D₂O-induced inhibition was) invariably observed in response to either glucose or sulfonylurea. This escape phenomenon supports the view that the inhibitory effect of D₂O on insulin)release is mainly due to a functional sideration of the microtubular-microfilamentous system. The present data suggest, therefore, that such a system is actively, involved in both phases of insulin release. (Endocrinology 95: 1518, 1974)

Footnotes

¹ Research Fellow of the NFWO (Brussels).

Received April 15, 1974.