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Localization of Immunoreactive Androgen in Testicular Tissue

⁺ Neuroendocrinology Research Section, Clarke Institute of Psychiatry Toronto, Canada, M5T 1R8
⁺⁺ University of Rochester, School of Medicine and Dentistry Rochester, New York 14642

Abstract

Immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. The bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horseradish peroxidase-diaminobenzidine technique. The highest concentration of androgen was found in the interstitial tissue but a significant amount was also present in the seminiferous tubules in the layer adjacent to the tubular wall. Very small amounts of androgen were localized in the tubular wall. This restricted localization in the tubules might indicate either de novo synthesis or active uptake of steroids intermediate in the biosynthesis of testosterone in the seminiferous tubules. (Endocrinology 96: 63, 1975)

Received May 9, 1974.