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Testosterone and 5-Reduced Androgen Secretion by Rabbit Testes-Epididymides Perfused In Vitro¹

Division of Reproductive Biology, Department of Population Dynamics, The Johns Hopkins University School of Hygiene and Public Health, and Department of Pharmacology and Experimental Therapeutics, The Johns Hopkins University School of Medicine Baltimore, Maryland 21205

Abstract

Mass spectrometric determinations confirmed that testosterone, 5-androstan-17βol-3-one, 5-androstan-3,17β-diol and 5-androstan-3β,17β-diol were present in venous effluent of in vitro perfused rabbit testes-epididymides. Testosterone, dihydrotestosterone, 3-androstanediol and 3β-androstanediol were secreted at 3.1 ± 0.9, 0.7 ± 0.2, 0.4 ± 0.1 and 0.6 ±0.1 5g/h when testes-epididymides were perfused with an artificial medium containing 2.5 ng/ml NIH-LH-S17, ovine. Surprisingly, testosterone constituted only 64% of the total mass of the four androgens secreted. These results probably reflect in vivo androgen secretion since hourly collections of spermatic vein blood from anesthetized rabbits contained 2.7 ± 0.9, 0.8 ± 0.3, 0.15 ± .04 and 0.5 ± 0.2 5g of testosterone, dihydrotestosterone, 3-androstanediol and 3β-androstanediol, respectively. Once again testosterone constituted only 64% of the total mass of the four androgens. (Endocrinology 96: 610, 1975)

Footnotes

¹ The research was presented in part at the Fifty- Sixth Annual Meeting of the Endocrine Society June 12-14, 1974. It was supported by U.S. Public Health Service Research Grant HD-07204 from the National Institute of Child Health and Human Development. The mass spectrometric studies were supported by Public Health Service Grant GM-16492.

² Barry Brown was a postdoctoral trainee in the Department of Population Dynamics supported by USPHS Training Grant Number HD-00109-07. FBI Laboratory, Federal Bureau of Investigation, 9th and Pennsylvania Avenue, Washington D.C. 20535.

³ Ian Jardine is a postdoctoral fellow in the Department of Pharmacology and Experimental Therapeutics, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205.

Received August 30, 1974.

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