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Measurement of Plasma and Tissue Triiodothyronine Concentration in the Rat by Radioimmunoassay¹

Departments of Physiology and Medicine, University of Connecticut School of Medicine, Farmington, Connecticut, The Medical Service, Veterans’ Administration Hospital Newington, Connecticut Medical Service, Hartford Hospital, Hartford, Connecticut, and the Endocrine Division, Department of Medicine, New England Medical Center Hospital Boston Massachusetts

Abstract

This paper reports a radioimmunoassay method for triiodothyronine (T₃) and the application of this assay to the study of plasma and tissue T₃ concentration in the rat. Several antisera formed to a T₃-bovine fibrogen complex in guinea pigs and T₃-thyroglobulin complex in rabbits are shown to have low or no cross reactivity with T_4, monoiodotyrosine, diiodotyrosine, tetraiodothyroacetic acid (TETRAC) and reverse T_3. Cross reactivity with T₃ derivatives, triiodothyroacetic acid (TRIAC), and triiodothyropropionic acid (TRIPROP) was variable, some antisera differentiating moderately well and others not at all. An extraction method is described which removes approximately 85% of added ¹²⁵I-T₃ or unlabelled T₃ from tissues and in the final step represents 57% of total tissue T_3. Mean plasma T₃ in 5 normal male rats was 58 ± 6.0 ng/100 ml (SEM), in liver 769 ± 56 ng/100 g, and kidney 627 ± 39 ng/100 g. Tissue to plasma concentration gradients for liver and kidney were 13.3 and 1.0.8, respectively. (Endociinology 96: 773, 1975)

Footnotes

¹ Supported by Grant No. AM 16684, U.S.P.H.S. and a grant from the Hartford Hospital.

Received August 5, 1974.