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Endocrinology, doi:10.1210/endo-96-3-773
Endocrinology Vol. 96, No. 3 773-780
Copyright © 1975 by the Endocrine Society.
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Measurement of Plasma and Tissue Triiodothyronine Concentration in the Rat by Radioimmunoassay1

IRADJ NEJAD, JUDITH BOLLINGER, MARY ANN MITNICK, PAUL SULLIVAN and SEYMOUR REICHLIN

Departments of Physiology and Medicine, University of Connecticut School of Medicine, Farmington, Connecticut, The Medical Service, Veterans’ Administration Hospital Newington, Connecticut Medical Service, Hartford Hospital, Hartford, Connecticut, and the Endocrine Division, Department of Medicine, New England Medical Center Hospital Boston Massachusetts

Abstract

This paper reports a radioimmunoassay method for triiodothyronine (T3) and the application of this assay to the study of plasma and tissue T3 concentration in the rat. Several antisera formed to a T3-bovine fibrogen complex in guinea pigs and T3-thyroglobulin complex in rabbits are shown to have low or no cross reactivity with T4, monoiodotyrosine, diiodotyrosine, tetraiodothyroacetic acid (TETRAC) and reverse T3. Cross reactivity with T3 derivatives, triiodothyroacetic acid (TRIAC), and triiodothyropropionic acid (TRIPROP) was variable, some antisera differentiating moderately well and others not at all. An extraction method is described which removes approximately 85% of added 125I-T3 or unlabelled T3 from tissues and in the final step represents 57% of total tissue T3. Mean plasma T3 in 5 normal male rats was 58 ± 6.0 ng/100 ml (SEM), in liver 769 ± 56 ng/100 g, and kidney 627 ± 39 ng/100 g. Tissue to plasma concentration gradients for liver and kidney were 13.3 and 1.0.8, respectively. (Endociinology 96: 773, 1975)

Footnotes

1 Supported by Grant No. AM 16684, U.S.P.H.S. and a grant from the Hartford Hospital.

Received August 5, 1974.






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Copyright © 1975 by The Endocrine Society