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A Short Method for the Isolation of Somatotrophs from the Rat Pituitary Gland^_*

G. SNYDER and W. C. HYMER

The Pennsylvania State University University Park Pennsylvania 16802

Abstract

Application of a density gradient centrifugation procedure for the isolation of somato-trophs from dispersed rat anterior pituitary cells is described. Two sequential discontinuous gradients of bovine serum albumin are used to enrich the somatotroph population from a frequency of 38% in the initial cell suspension to a final purity of 85%. The isolated somatotrophs retain both hisrochemioal and ultrastructural integrity throughout the isolation procedure. The isolated somatotrophs also release; immunoassayable growth hormone when incubated with 6 HIM dibutyryl 3',5'-eyclic AMP. The method offers the advantages of being both simple and short. (Endocrinology 96: 792, 1975)

Footnotes

^_* This work was supported by NSF Grant GB-33686.

Recipient of PHS Research Career Development Award K 05-AM-15808.

Received July 29, 1974.