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Endocrinology, Vol 96, 796-798, Copyright © 1975 by Endocrine Society

ARTICLES

A direct radioimmunoassay of corticosterone in rat serum

C Gomez-Sanchez, BA Murry, DC Kem and NM Kaplan

A direct radioimmunoassay of corticosterone is described requiring as little as 1-2 mul of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.




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Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1975 by The Endocrine Society