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Endocrinology, doi:10.1210/endo-96-3-796
Endocrinology Vol. 96, No. 3 796-798
Copyright © 1975 by the Endocrine Society.
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A Direct Radioimmunoassay of Corticosterone in Rat Serum

CELSO GOMEZ-SANCHEZ, BARBARA A. MURRY, DAVID C. KEM and NORMAN M. KAPLAN

Department of Internal Medicine, The University of Texas Southwestern Medical School Dallas, Texas 75235

Abstract

A direct radioimmunoassay of cortico-sterone is described requiring as little as 1–2 µl of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat (Endocrinology 96: 796, 1975)

Received July 15, 1974.






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Copyright © 1975 by The Endocrine Society