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Enzymatic Oxidation and Reduction of C₁₉-⁵-3β-Hydroxysteroids by Hepatic Microsomes. IV. Induction of DHA Hydroxylases and Aminopyrine N-Demethylase in Immature Male Rats by Androgens

Department of Obstetrics and Gynecology, University of Washington School of Medicine Seattle, Washington 98195

Abstract

The capacities of various C¹⁹ steroids for prematurely inducing the normal metabolic patterns of rat liver in adulthood (70 days old) have been studied with hepatic microsomes of 42-day-old males castrated on day 24, 30, 32, or 34 of life. Dehydroepiandrosterone 16-hydroxylase activity was significantly reduced (P < 0.05) by castration during this 10-day interval but the 7- and 7β- hydroxylases, the N-demethylation of aminopyrine, and cytochrome P-450 concentration were unaffected. Daily administration of testosterone stimulated the DHA 16- and 7β-hydroxylases, aminopyrine N-demethylation, and increased the P-450 content, but suppressed the 7-hydroxylase. These effects only appeared with more than 1 week of the continuous treatment. Testosterone was the most active of the androgens studied; dihydrotestosterone (DHT) increased the DHA 16-hydroxylase to a lesser extent, but this steroid and etiocholanolone stimulated DHA 7-hydroxylation; androsterone was totally ineffective. These data suggest that testosterone rather than DHT from pubertal testes plays a significant role in control of hepatic oxidative enzyme activities during puberty. (Endocrinology 96: 815, 1975)

Received April 15, 1974.