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The Department of Pharmacology Neio York University School of Medicine New York, New York 10016 The Biology Department, Brookhaven National Laboratory Upton, New York 11973
Abstract
A sensitive radioimmunoassay is described for the measurement of plasma concentrations of canine growth hormone (cGH) as low as 0.25 ng/ml. The assay utilizes enzymatically iodinated cGH and the double antibody technique. The mean plasma concentration of growth hormone in the normal dog after overnight fast is 1.75 ± .17 ng/ml. Exogenous cGH was cleared from the plasma of both the normal and hypophysectomized dog with a mean half-life of 25.6 ± 1.0 min and was distributed in a volume equal to 8.9% of the body weight. Insulin hypoglycemia produced a 3- to 5-fold increase in plasma GH in 4 of 6 dogs and arginine infusion failed to produce a statistically significant rise. (Endocrinology 96: 822, 1975)
Footnotes
1 Investigations carried out at New York University School of Medicine were supported by Research Grant AM 10188 from the National Institute of Arthritis and Metabolic Diseases and by Grant P-207 (H) from the American Cancer Society. Part of this research was carried out at Brookhaven National Laboratory under the auspices of the U.S. Atomic Energy Commission. A preliminary account of this work was presented during the 57th Annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N.J., April, 1973.
2 Dr. Steele died during the course of this study, on Jan. 18, 1974. He was a devoted scientist and warm friend and we dedicate this presentation to his memory.
Received July 10, 1974.
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