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Endocrinology, doi:10.1210/endo-96-3-822
Endocrinology Vol. 96, No. 3 822-828
Copyright © 1975 by the Endocrine Society.
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Radioimmunoassay of Canine Growth Hormone: Enzymatic Radioiodination1

J. HAMPSHIRE, N. ALTSZULER, R. STEELE2 and L.J. GREENE

The Department of Pharmacology Neio York University School of Medicine New York, New York 10016 The Biology Department, Brookhaven National Laboratory Upton, New York 11973

Abstract

A sensitive radioimmunoassay is described for the measurement of plasma concentrations of canine growth hormone (cGH) as low as 0.25 ng/ml. The assay utilizes enzymatically iodinated cGH and the double antibody technique. The mean plasma concentration of growth hormone in the normal dog after overnight fast is 1.75 ± .17 ng/ml. Exogenous cGH was cleared from the plasma of both the normal and hypophysectomized dog with a mean half-life of 25.6 ± 1.0 min and was distributed in a volume equal to 8.9% of the body weight. Insulin hypoglycemia produced a 3- to 5-fold increase in plasma GH in 4 of 6 dogs and arginine infusion failed to produce a statistically significant rise. (Endocrinology 96: 822, 1975)

Footnotes

1 Investigations carried out at New York University School of Medicine were supported by Research Grant AM 10188 from the National Institute of Arthritis and Metabolic Diseases and by Grant P-207 (H) from the American Cancer Society. Part of this research was carried out at Brookhaven National Laboratory under the auspices of the U.S. Atomic Energy Commission. A preliminary account of this work was presented during the 57th Annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N.J., April, 1973.

2 Dr. Steele died during the course of this study, on Jan. 18, 1974. He was a devoted scientist and warm friend and we dedicate this presentation to his memory.

Received July 10, 1974.






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Copyright © 1975 by The Endocrine Society