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Endocrinology, Vol 96, 897-889, Copyright © 1975 by Endocrine Society

ARTICLES

Cellular localization of 5alpha-reductase and 3alpha-hydroxysteroid dehydrogenase in the seminiferous tubule of the rat testis

JH Dorrington and IB Fritz

Seminiferous tubules isolated from normal adult rats converted (14C) testosterone to (14C) androstanediol and (14C) androstenedione as the major metabolites; (14C) dihydrotestosterone and (14C) androsterone were produced in lesser amounts. Tubules from immature rats (26-28 days of age) converted a higher proportion of (14C) testosterone to 5alpha- reduced products than did tubules from adult rats. Spermatocyte- enriched preparations contain 5alpha-reductase. The lower level of 5alpha-reductase activity in spermatid-spermatocyte preparations indicates that this enzyme is low or absent in spermatids. Sertoli cell- enriched preparations contain 5 alpha-reductase and a high level of 3alpha-hydroxysteroid dehydrogenase. The results show that spermatocytes and Sertoli cells have the capacity to metabolise (14C) testosterone to 5alpha-reduced products; dihydrotesterone is the major product formed by spermatocytes, whereas in Sertoli cells further metabolism to 5alpha-androstane-3alpha, 17beta-diol occurs.




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Copyright © 1975 by The Endocrine Society