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Stimulation of Division of Yl Adrenal Cells by a Growth Factor Isolated from Bovine Pituitary Glands

The Salk Institute for Biological Studies, PO Box 1809 San Die Diego, California 92112

Abstract

The effect of Fibroblast Growth Factor (FGF) on the initiation of DNA synthesis and on the rate of division of the Yl adrenal cell line has been investigated. In sparse populations ofYl cells (4 x 10³cells/cm²) maintained in 0.2% calf serum, FGF was able to initiate DNA synthesis to the same extent that an optimal concentration of serum could. Cells maintained in 0.2% calf serum sustained continuous growth when given 5 ng/ml of FGF daily. Cultures fixed and stained with crystal violet showed FGF colonies to be of equivalent size and quantity as those with serum alone. Insulin had no mitogenic activity of itsown at concentrations as high as 500 ng/ml nor did it have any potentiating effect on themitogenic activity of FGF. Glucocorticoids (0.1 µg/ml to 1 µg/ml) inhibited (25%) the initiation of DNA synthesis as well as the rate of division induced by FGFACTH (0.75 IU/ml) was clearly inhibitory. Not only did it reduce the rate of division of cellsin serum but it also reduced the rate of DNA synthesis and inhibited division in the presence of FGF. (Endocrinology 97: 102, 1975)

Received October 31, 1974.

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