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Endocrinology, Vol 97, 621-629, Copyright © 1975 by Endocrine Society

ARTICLES

The purification and characterization of rat placental lactogen

MC Roberston and HG Friesen

A method has been developed for the purification of rat placental lactogen employing a specific radioreceptor assay (RRA). The method involves precipitation with ammonium sulfate, gel filtration on Sephadex G-100, ion-exchange chromatography on DEAE-cellulose and CM- Sephadex, and preparative isoelectric focusing. The isolation precedure results in a 1300-fold purification and a 10% yield of rat placental lactogen. Potency estimates by RRA indicate that the purified hormone is 41% as active as the ovine prolactin standard (25 I U/mg), but 169% as active as the NIH human placental lactogen preparation. Polyacrylamide gel electrophoresis at pH 8.9 annd analytical isoelectric focusing of the rat placental lactogen reveal 2 major and 2 minor components, all of which are active in the RRA.


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D. O. Wiemers, L.-j. Shao, R. Ain, G. Dai, and M. J. Soares
The Mouse Prolactin Gene Family Locus
Endocrinology, January 1, 2003; 144(1): 313 - 325.
[Abstract] [Full Text] [PDF]


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