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Nuclear Localization of Insulin-Like Growth Factor Binding Protein 3 in a Lung Cancer Cell Line

Department of Internal Medicine (G.J., K.N., B.W., S.W., K.H.), Division of Hematology/Oncology, Philipps-University, Baldingerstrasse, D-35033 Marburg, Germany; Molecular Concepts Research (MCR) (R.T.R.), Munich, Germany; and I.M. Setchenow Medical Academy (E.K.), Moscow

Address all correspondence and requests for reprints to: Gabriele Jacques, Klinikum de Philipps-Universitat, Zentrum fur Innere Medizin, Baldinger Strasse, Marburg, 35043 Germany.

	Abstract

Top Abstract

 
Considerable evidence exists that lung cancer cell lines produce large amounts of insulin-like growth factor-binding proteins (IGFBPs). In addition, these cells are subject to an autocrine or paracrine growth control by insulin-like growth factors (IGFs). We now demonstrate by immunocytochemistry with IGFBP-3 antibodies that nuclei of a lung cancer cell line distinctly immunostain for IGFBP-3. This finding led us to investigate in more detail the localization of this protein that, to date, had only been known to occur extracellularly. Ligand blotting revealed that purified nuclear extracts contain a 43,000-Da IGFBP which can bind [I¹²⁵]IGF-I. By Western blot this protein was identified as IGFBP-3. Thus, our data are consistent with the results of a previous structural study predicting a nuclear localization for IGFBP-3. Moreover, our findings raise the possibility that nuclear IGFBP-3 is functional and involved in the pathogenesis of lung cancer.

Received October 21, 1996.

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This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jaques, G. Articles by Havemann, K. Search for Related Content PubMed PubMed Citation Articles by Jaques, G. Articles by Havemann, K.