This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carro, E. Articles by Dieguez, C. Search for Related Content PubMed PubMed Citation Articles by Carro, E. Articles by Dieguez, C.

Regulation of in Vivo Growth Hormone Secretion by Leptin

Department of Physiology (E.C., R.S., C.D.) and Department of Medicine, Endocrine Area Complejo Hospitalario Universitario de Santiago (F.E.C.), University of Santiago, Santiago de Compostela Spain; Department of Medicine (R.V.C.), Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania

Address correspondence and requests for reprints to: Dr. C. Dieguez, P.O. Box 563, Santiago de Compostela, 15700 Spain.

	Abstract

Top Abstract

 
Leptin, the product of the ob gene, is a recently discovered hormone secreted by adipocytes that regulates food intake and energy expenditure. Growth hormone (GH) secretion is markedly influenced by body weight being markedly suppressed in obesity and underweight. The aim of the present study was to study whether leptin can act as a metabolic signal connecting the adipose tissue with the growth hormone axis. We administered leptin antiserum (10 ul, i.c.v.) or normal rabbit serum (NRS; 10 ul, i.c.v.) to freely moving fed rats. Furthermore we assessed the effect of leptin administration (10 ug, i.c.v.) on fed and fasted rats. Spontaneous GH secretion was assessed over 6 hours with blood samples taken every 15 min. Administration of leptin antiserum led to a decrease in spontaneous GH secretion as assessed by the area under the curve (AUC) (168 ± 72 ng/ml/6h) in comparison to NRS-treated rats (813 ± 179 ng/ml/6 h, p < 0.01). While leptin administration (10 ug/rat; i.c.v.) to normal fed rats did not modify spontaneous GH secretion, leptin administration to fasted rats led to a reversal of the inhibitory effect exerted by fasting on GH secretion (AUC, 1650 ± 351 ng/ml.6 h vs 77 ± 32 ng/ml/6 h, p < 0.01). This data suggests that leptin is a metabolic signal that regulates GH secretion.

Received January 27, 1997.

This article has been cited by other articles:

				M. W. Hamrick Leptin and Bone: A Consensus Emerging? IBMS BoneKEy, March 1, 2007; 4(3): 99 - 107. [Abstract] [Full Text] [PDF]

				R. M. Luque, Z. H. Huang, B. Shah, T. Mazzone, and R. D. Kineman Effects of leptin replacement on hypothalamic-pituitary growth hormone axis function and circulating ghrelin levels in ob/ob mice Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E891 - E899. [Abstract] [Full Text] [PDF]

				P. R Buff, N. T Messer IV, A. M Cogswell, D. A Wilson, P. J Johnson, D. H Keisler, and V. K Ganjam Induction of pulsatile secretion of leptin in horses following thyroidectomy J. Endocrinol., February 1, 2007; 192(2): 353 - 359. [Abstract] [Full Text] [PDF]

				F. Rodriguez-Pacheco, A. J. Martinez-Fuentes, S. Tovar, L. Pinilla, M. Tena-Sempere, C. Dieguez, J. P. Castano, and M. M. Malagon Regulation of Pituitary Cell Function by Adiponectin Endocrinology, January 1, 2007; 148(1): 401 - 410. [Abstract] [Full Text] [PDF]

				C R. Barb, R. R Kraeling, G. B Rampacek, and G. J Hausman The role of neuropeptide Y and interaction with leptin in regulating feed intake and luteinizing hormone and growth hormone secretion in the pig. Reproduction, June 1, 2006; 131(6): 1127 - 1135. [Abstract] [Full Text] [PDF]

				R. M. Luque and R. D. Kineman Impact of Obesity on the Growth Hormone Axis: Evidence for a Direct Inhibitory Effect of Hyperinsulinemia on Pituitary Function Endocrinology, June 1, 2006; 147(6): 2754 - 2763. [Abstract] [Full Text] [PDF]

				K. A. Clark, S. M. J. MohanKumar, B. S. Kasturi, and P. S. MohanKumar Effects of central and systemic administration of leptin on neurotransmitter concentrations in specific areas of the hypothalamus Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R306 - R312. [Abstract] [Full Text] [PDF]

				P. R. Buff, C. D. Morrison, V. K. Ganjam, and D. H. Keisler Effects of short-term feed deprivation and melatonin implants on circadian patterns of leptin in the horse J Anim Sci, May 1, 2005; 83(5): 1023 - 1032. [Abstract] [Full Text] [PDF]

				C. F. Liew, S. D. Wise, K. P. Yeo, and K. O. Lee Insulin-Like Growth Factor Binding Protein-1 Is Independently Affected by Ethnicity, Insulin Sensitivity, and Leptin in Healthy, Glucose-Tolerant Young Men J. Clin. Endocrinol. Metab., March 1, 2005; 90(3): 1483 - 1488. [Abstract] [Full Text] [PDF]

				G Tulipano, A V Vergoni, D Soldi, E E Muller, and D Cocchi Characterization of the resistance to the anorectic and endocrine effects of leptin in obesity-prone and obesity-resistant rats fed a high-fat diet J. Endocrinol., November 1, 2004; 183(2): 289 - 298. [Abstract] [Full Text] [PDF]

				G. Gat-Yablonski, T. Ben-Ari, B. Shtaif, O. Potievsky, O. Moran, R. Eshet, G. Maor, Y. Segev, and M. Phillip Leptin Reverses the Inhibitory Effect of Caloric Restriction on Longitudinal Growth Endocrinology, January 1, 2004; 145(1): 343 - 350. [Abstract] [Full Text] [PDF]

				L. GHIZZONI and G. MASTORAKOS Interactions of Leptin, GH, and Cortisol in Normal Children Ann. N.Y. Acad. Sci., November 1, 2003; 997(1): 56 - 63. [Abstract] [Full Text] [PDF]

				P. L. Roubertoux, I. Le Roy, S. Tordjman, A. Cherfou, and D. Migliore-Samour Analysis of Quantitative Trait Loci for Behavioral Laterality in Mice Genetics, March 1, 2003; 163(3): 1023 - 1030. [Abstract] [Full Text] [PDF]

				L. M. Seoane, M. Lopez, S. Tovar, F. F. Casanueva, R. Senaris, and C. Dieguez Agouti-Related Peptide, Neuropeptide Y, and Somatostatin-Producing Neurons Are Targets for Ghrelin Actions in the Rat Hypothalamus Endocrinology, February 1, 2003; 144(2): 544 - 551. [Abstract] [Full Text] [PDF]

				M. Ozata, C. Dieguez, and F. F. Casanueva The Inhibition of Growth Hormone Secretion Presented in Obesity Is Not Mediated by the High Leptin Levels: A Study in Human Leptin Deficiency Patients J. Clin. Endocrinol. Metab., January 1, 2003; 88(1): 312 - 316. [Abstract] [Full Text] [PDF]

				M. L. Barker-Gibb, A. Sahu, C. R. Pohl, and T. M. Plant Elevating Circulating Leptin in Prepubertal Male Rhesus Monkeys (Macaca mulatta) Does Not Elicit Precocious Gonadotropin-Releasing Hormone Release, Assessed Indirectly J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 4976 - 4983. [Abstract] [Full Text] [PDF]

				S. Khosla Editorial: Leptin--Central or Peripheral to the Regulation of Bone Metabolism? Endocrinology, November 1, 2002; 143(11): 4161 - 4164. [Full Text] [PDF]

				H. Watanobe and S. Habu Leptin Regulates Growth Hormone-Releasing Factor, Somatostatin, and alpha -Melanocyte-Stimulating Hormone But Not Neuropeptide Y Release in Rat Hypothalamus In Vivo: Relation with Growth Hormone Secretion J. Neurosci., July 15, 2002; 22(14): 6265 - 6271. [Abstract] [Full Text] [PDF]

				J. Z. Kasa-Vubu, A. Barkan, P. Olton, T. Meckmongkol, N. E. Carlson, and C. M. Foster Incomplete Modified Fast in Obese Early Pubertal Girls Leads to an Increase in 24-Hour Growth Hormone Concentration and a Lessening of the Circadian Pattern in Leptin J. Clin. Endocrinol. Metab., April 1, 2002; 87(4): 1885 - 1893. [Abstract] [Full Text] [PDF]

				H. S. Randeva, R. D. Murray, K. C. Lewandowski, C. J. O'Callaghan, R. Horn, P. O'Hare, G. Brabant, E. W. Hillhouse, and S. M. Shalet Differential Effects of GH Replacement on the Components of the Leptin System in GH-Deficient Individuals J. Clin. Endocrinol. Metab., February 1, 2002; 87(2): 798 - 804. [Abstract] [Full Text] [PDF]

				M. Baratta, R. Saleri, G. L. Mainardi, D. Valle, A. Giustina, and C. Tamanini Leptin Regulates GH Gene Expression and Secretion and Nitric Oxide Production in Pig Pituitary Cells Endocrinology, February 1, 2002; 143(2): 551 - 557. [Abstract] [Full Text] [PDF]

				R. Coutant, F. Boux de Casson, O. Douay, E. Mathieu, S. Rouleau, F. Beringue, P. Gillard, J. M. Limal, and P. Descamps Relationships between Placental GH Concentration and Maternal Smoking, Newborn Gender, and Maternal Leptin: Possible Implications for Birth Weight J. Clin. Endocrinol. Metab., October 1, 2001; 86(10): 4854 - 4859. [Abstract] [Full Text] [PDF]

				A. M. Ahmad, R. Guzder, A. M. Wallace, J. Thomas, W. D. Fraser, and J. P. Vora Circadian and Ultradian Rhythm and Leptin Pulsatility in Adult GH Deficiency: Effects of GH Replacement J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3499 - 3506. [Abstract] [Full Text] [PDF]

				D. Le Roith, C. Bondy, S. Yakar, J.-L. Liu, and A. Butler The Somatomedin Hypothesis: 2001 Endocr. Rev., February 1, 2001; 22(1): 53 - 74. [Abstract] [Full Text]

				H. Christou, J. M. Connors, M. Ziotopoulou, V. Hatzidakis, E. Papathanassoglou, S. A. Ringer, and C. S. Mantzoros Cord Blood Leptin and Insulin-Like Growth Factor Levels are Independent Predictors of Fetal Growth J. Clin. Endocrinol. Metab., February 1, 2001; 86(2): 935 - 938. [Abstract] [Full Text]

				R. Heptulla, A. Smitten, B. Teague, W. V. Tamborlane, Y.-Z. Ma, and S. Caprio Temporal Patterns of Circulating Leptin Levels in Lean and Obese Adolescents: Relationships to Insulin, Growth Hormone, and Free Fatty Acids Rhythmicity J. Clin. Endocrinol. Metab., January 1, 2001; 86(1): 90 - 96. [Abstract] [Full Text]

				L Bowles and P Kopelman Leptin: of mice and men? J. Clin. Pathol., January 1, 2001; 54(1): 1 - 3. [Abstract] [Full Text] [PDF]

				J.-L. Wang, N. Chinookoswong, S. Yin, and Z.-Q. Shi Calorigenic actions of leptin are additive to, but not dependent on, those of thyroid hormones Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1278 - E1285. [Abstract] [Full Text] [PDF]

				S. Nagatani, Y. Zeng, D. H. Keisler, D. L. Foster, and C. A. Jaffe Leptin Regulates Pulsatile Luteinizing Hormone and Growth Hormone Secretion in the Sheep Endocrinology, November 1, 2000; 141(11): 3965 - 3975. [Abstract] [Full Text] [PDF]

				G. GARIBOTTO, A. BARRECA, A. SOFIA, R. RUSSO, F. FIORINI, G. CAPPELLI, F. CAVATORTA, A. CESARONE, R. FRANCESCHINI, P. SACCO, et al. Effects of Growth Hormone on Leptin Metabolism and Energy Expenditure in Hemodialysis Patients with Protein-Calorie Malnutrition J. Am. Soc. Nephrol., November 1, 2000; 11(11): 2106 - 2113. [Abstract] [Full Text]

				S. Vidal, S. M. Cohen, E. Horvath, K. Kovacs, B. W. Scheithauer, B. G. Burguera, and R. V. Lloyd Subcellular Localization of Leptin in Non-tumorous and Adenomatous Human Pituitaries: An Immuno-ultrastructural Study J. Histochem. Cytochem., August 1, 2000; 48(8): 1147 - 1152. [Abstract] [Full Text]

				M. Maccario, G. Aimaretti, G. Corneli, C. Gauna, S. Grottoli, M. Bidlingmaier, C. J. Strasburger, C. Dieguez, F. F. Casanueva, and E. Ghigo Short-term fasting abolishes the sex-related difference in GH and leptin secretion in humans Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E411 - E416. [Abstract] [Full Text] [PDF]

				M. B. HORLICK, M. ROSENBAUM, M. NICOLSON, L. S. LEVINE, B. FEDUN, J. WANG, R. N. PIERSON Jr., and R. L. LEIBEL Effect of Puberty on the Relationship between Circulating Leptin and Body Composition J. Clin. Endocrinol. Metab., July 1, 2000; 85(7): 2509 - 2518. [Abstract] [Full Text]

				C. S. MANTZOROS Role of Leptin in Reproduction Ann. N.Y. Acad. Sci., April 1, 2000; 900(1): 174 - 183. [Abstract] [Full Text] [PDF]

				J. Iqbal, S. Pompolo, R. V. Considine, and I. J. Clarke Localization of Leptin Receptor-Like Immunoreactivity in the Corticotropes, Somatotropes, and Gonadotropes in the Ovine Anterior Pituitary Endocrinology, April 1, 2000; 141(4): 1515 - 1520. [Abstract] [Full Text] [PDF]

				K. J. Suter, C. R. Pohl, and M. E. Wilson Circulating Concentrations of Nocturnal Leptin, Growth Hormone, and Insulin-Like Growth Factor-I Increase before the Onset of Puberty in Agonadal Male Monkeys: Potential Signals for the Initiation of Puberty J. Clin. Endocrinol. Metab., February 1, 2000; 85(2): 808 - 814. [Abstract] [Full Text]

				J. S. Torgerson, B. Carlsson, K. Stenlöf, L. M. S. Carlsson, E. Bringman, and L. Sjöström A Low Serum Leptin Level at Baseline and a Large Early Decline in Leptin Predict a Large 1-Year Weight Reduction in Energy-Restricted Obese Humans J. Clin. Endocrinol. Metab., November 1, 1999; 84(11): 4197 - 4203. [Abstract] [Full Text]

				H. Fors, H. Matsuoka, I. Bosaeus, S. Rosberg, K. A. Wikland, and R. Bjarnason Serum Leptin Levels Correlate with Growth Hormone Secretion and Body Fat in Children J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3586 - 3590. [Abstract] [Full Text] [PDF]

				L. Jin, B. G. Burguera, M. E. Couce, B. W. Scheithauer, J. Lamsan, N. L. Eberhardt, E. Kulig, and R. V. Lloyd Leptin and Leptin Receptor Expression in Normal and Neoplastic Human Pituitary: Evidence of a Regulatory Role for Leptin on Pituitary Cell Proliferation J. Clin. Endocrinol. Metab., August 1, 1999; 84(8): 2903 - 2911. [Abstract] [Full Text]

				A. Cai and J. F. Hyde The Human Growth Hormone-Releasing Hormone Transgenic Mouse as a Model of Modest Obesity: Differential Changes in Leptin Receptor (OBR) Gene Expression in the Anterior Pituitary and Hypothalamus after Fasting and OBR Localization in Somatotrophs Endocrinology, August 1, 1999; 140(8): 3609 - 3614. [Abstract] [Full Text]

				A. M. Watson, S. M. Poloyac, G. Howard, and R. A. Blouin Effect of Leptin on Cytochrome P-450, Conjugation, and Antioxidant Enzymes in the ob/ob Mouse Drug Metab. Dispos., June 1, 1999; 27(6): 695 - 700. [Abstract] [Full Text]

				R. K. Støving, J. D. Veldhuis, A. Flyvbjerg, J. Vinten, J. Hangaard, O. G. Koldkjær, J. Kristiansen, and C. Hagen Jointly Amplified Basal and Pulsatile Growth Hormone (GH) Secretion and Increased Process Irregularity in Women with Anorexia Nervosa: Indirect Evidence for Disruption of Feedback Regulation within the GH-Insulin-Like Growth Factor I Axis J. Clin. Endocrinol. Metab., June 1, 1999; 84(6): 2056 - 2063. [Abstract] [Full Text]

				R. S. Ahima, C. Bjorbaek, S. Osei, and J. S. Flier Regulation of Neuronal and Glial Proteins by Leptin: Implications for Brain Development Endocrinology, June 1, 1999; 140(6): 2755 - 2762. [Abstract] [Full Text]

				C. S. Mantzoros The Role of Leptin in Human Obesity and Disease: A Review of Current Evidence Ann Intern Med, April 20, 1999; 130(8): 671 - 680. [Abstract] [Full Text] [PDF]

				E. E. Muller, V. Locatelli, and D. Cocchi Neuroendocrine Control of Growth Hormone Secretion Physiol Rev, April 1, 1999; 79(2): 511 - 607. [Abstract] [Full Text] [PDF]

				B. A. Henry, J. W. Goding, W. S. Alexander, A. J. Tilbrook, B. J. Canny, F. Dunshea, A. Rao, A. Mansell, and I. J. Clarke Central Administration of Leptin to Ovariectomized Ewes Inhibits Food Intake without Affecting the Secretion of Hormones from the Pituitary Gland: Evidence for a Dissociation of Effects on Appetite and Neuroendocrine Function Endocrinology, March 1, 1999; 140(3): 1175 - 1182. [Abstract] [Full Text]

				R. B. S. Harris Parabiosis between db/db and ob/ob or db/+ Mice Endocrinology, January 1, 1999; 140(1): 138 - 145. [Abstract] [Full Text]

				I. Shimon, X. Yan, D. A. Magoffin, T. C. Friedman, and S. Melmed Intact Leptin Receptor Is Selectively Expressed in Human Fetal Pituitary and Pituitary Adenomas and Signals Human Fetal Pituitary Growth Hormone Secretion J. Clin. Endocrinol. Metab., November 1, 1998; 83(11): 4059 - 4064. [Abstract] [Full Text]

				M. Miyakawa, T. Tsushima, H. Murakami, O. Isozaki, H. Demura, and T. Tanaka Effect of Growth Hormone (GH) on Serum Concentrations of Leptin: Study in Patients with Acromegaly and GH Deficiency J. Clin. Endocrinol. Metab., October 1, 1998; 83(10): 3476 - 3479. [Abstract] [Full Text]

				G. Van den Berghe, P. Wouters, L. Carlsson, R. C. Baxter, R. Bouillon, and C. Y. Bowers Leptin Levels in Protracted Critical Illness: Effects of Growth Hormone-Secretagogues and Thyrotropin-Releasing Hormone J. Clin. Endocrinol. Metab., September 1, 1998; 83(9): 3062 - 3070. [Abstract] [Full Text]

				G. S. Tannenbaum, W. Gurd, and M. Lapointe Leptin Is a Potent Stimulator of Spontaneous Pulsatile Growth Hormone (GH) Secretion and the GH Response to GH-Releasing Hormone Endocrinology, September 1, 1998; 139(9): 3871 - 3875. [Abstract] [Full Text] [PDF]

				B. Kriström, B. Carlsson, S. Rosberg, L. M. S. Carlsson, and K. Albertsson-Wikland Short-Term Changes in Serum Leptin Levels Provide a Strong Metabolic Marker for the Growth Response to Growth Hormone Treatment in Children J. Clin. Endocrinol. Metab., August 1, 1998; 83(8): 2735 - 2741. [Abstract] [Full Text]

				M. A. Llopis, M. L. Granada, G. Cuatrecasas, X. Formiguera, L. Sánchez-Planell, A. SanmartÍ, A. Alastrué, M. Rull, A. Corominas, and M. Foz Growth Hormone-Binding Protein Directly Depends on Serum Leptin Levels in Adults with Different Nutritional Status J. Clin. Endocrinol. Metab., June 1, 1998; 83(6): 2006 - 2011. [Abstract] [Full Text]

				X. Casabiell, V. Piñeiro, R. Peino, M. Lage, J. Camiña, R. Gallego, L. G. Vallejo, C. Dieguez, and F. F. Casanueva Gender Differences in Both Spontaneous and Stimulated Leptin Secretion by Human Omental Adipose Tissue in Vitro: Dexamethasone and Estradiol Stimulate Leptin Release in Women, But Not in Men J. Clin. Endocrinol. Metab., June 1, 1998; 83(6): 2149 - 2155. [Abstract] [Full Text]

				J. S. Flier What's in a Name? In Search of Leptin's Physiologic Role J. Clin. Endocrinol. Metab., May 1, 1998; 83(5): 1407 - 1413. [Full Text]

				E. D. Eckert, C. Pomeroy, N. Raymond, P. F. Kohler, P. Thuras, and C. Y. Bowers Leptin in Anorexia Nervosa J. Clin. Endocrinol. Metab., March 1, 1998; 83(3): 791 - 795. [Abstract] [Full Text]

This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar