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Regulation of in Vivo Growth Hormone Secretion by Leptin

Department of Physiology (E.C., R.S., C.D.) and Department of Medicine, Endocrine Area Complejo Hospitalario Universitario de Santiago (F.E.C.), University of Santiago, Santiago de Compostela Spain; Department of Medicine (R.V.C.), Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania

Address correspondence and requests for reprints to: Dr. C. Dieguez, P.O. Box 563, Santiago de Compostela, 15700 Spain.

	Abstract

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Leptin, the product of the ob gene, is a recently discovered hormone secreted by adipocytes that regulates food intake and energy expenditure. Growth hormone (GH) secretion is markedly influenced by body weight being markedly suppressed in obesity and underweight. The aim of the present study was to study whether leptin can act as a metabolic signal connecting the adipose tissue with the growth hormone axis. We administered leptin antiserum (10 ul, i.c.v.) or normal rabbit serum (NRS; 10 ul, i.c.v.) to freely moving fed rats. Furthermore we assessed the effect of leptin administration (10 ug, i.c.v.) on fed and fasted rats. Spontaneous GH secretion was assessed over 6 hours with blood samples taken every 15 min. Administration of leptin antiserum led to a decrease in spontaneous GH secretion as assessed by the area under the curve (AUC) (168 ± 72 ng/ml/6h) in comparison to NRS-treated rats (813 ± 179 ng/ml/6 h, p < 0.01). While leptin administration (10 ug/rat; i.c.v.) to normal fed rats did not modify spontaneous GH secretion, leptin administration to fasted rats led to a reversal of the inhibitory effect exerted by fasting on GH secretion (AUC, 1650 ± 351 ng/ml.6 h vs 77 ± 32 ng/ml/6 h, p < 0.01). This data suggests that leptin is a metabolic signal that regulates GH secretion.

Received January 27, 1997.

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