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Endocrinology Vol. 139, No. 11 4743
Copyright © 1998 by The Endocrine Society


ARTICLES

Osteotropic Agents Regulate the Expression of Osteoclast Differentiation Factor and Osteoprotegerin in Osteoblastic Stromal Cells

Nicole J. Horwood, Jan Elliott, T. John Martin and Matthew T. Gillespie

St. Vincent’s Institute of Medical Research and The University of Melbourne, Department of Medicine, St. Vincent’s Hospital, Fitzroy, Victoria 3065, Australia

Address all correspondence and requests for reprints to: Matthew T. Gillespie, St. Vincent’s Institute of Medical Research, The University of Melbourne, 41 Victoria Parade, Fitzroy 3065, Australia. E-mail: m.gillespie{at}medicine.unimelb.edu.au


    Abstract
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 Abstract
 
The osteoclastogenic factor of osteoblastic origin has recently been elucidated as a novel Tumor Necrosis Factor (TNF)-ligand family member, termed osteoclast differentiation factor (ODF). Using a semiquantitative RT-PCR approach, we sought to determine the mRNA expression of ODF and its decoy receptor, osteoprotegerin (OPG), in a selection of osteoblastic cell lines and in response to three factors representative of different signal transduction pathways, vitamin D receptor, protein kinase A or gp130. Each osteotropic agent, either 1,25-(OH)2D3, PTH or IL-11, promoted an increase in the ratio of ODF:OPG, with maximal stimulation occurring at 24h, 4h, and 8h, respectively, and furthermore each was shown to act in a dose-dependent manner. This report establishes that osteoblastic cell lines incapable of supporting osteoclast formation have markedly reduced ODF expression and also illustrates the importance of the relative abundance of ODF compared with the levels of OPG for the induction of osteoclastogenesis.

Received May 13, 1998.




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