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Pregnancy-Specific Protein B Induces Release of an Alpha Chemokine in Bovine Endometrium

Reproductive Biology Program (K.J.A. J.E.V., T.R.H.), Department of Animal Science, University of Wyoming, Laramie, Wyoming 82071; and Departments of Animal and Veterinary Science (C.P.K., R.G.S.), University of Idaho, Moscow, Idaho 83843

Address all correspondence and requests for reprints to: Thomas R. Hansen, Ph.D., University of Wyoming, Department of Animal Science, College of Agriculture, P. O. Box 3684, Laramie, Wyoming 82071-3684.

	Abstract

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Pregnancy-specific protein B (PSPB), is secreted from binucleate trophoblast of the bovine conceptus as early as day 15 of pregnancy. The objective of this experiment was to determine if PSPB induced uterine proteins. PSPB was purified from day 120 cotyledons using antibody-based affinity chromatography. Endometrium from day 14 non-pregnant cows (n = 3) was prepared for explant (³H-Leu added) culture. Radiolabeled proteins released into medium were dialyzed, separated using 1D-PAGE, and detected using fluorography and densitometry. PSPB (0, 0.5, 5, 25 & 50 nM) caused a concentration-dependent increase in the release of a radiolabeled 8-kDa uterine protein. Western blots revealed that the 8-kDa protein cross-reacted with antibody against granulocyte chemotactic protein-2 (GCP-2). PSPB also induced release of GCP-2 by bovine endometrial (BEND) cells in primary culture. The induction of GCP-2 by PSPB was blocked by addition of antiserum against PSPB (1:4 molar ratio). This is the first indication that PSPB has a hormonal role in inducing GCP-2, an alpha chemokine that also is induced by interferon-tau during early pregnancy. This chemotactic cytokine may be integral to mediating adhesion, inflammation and angiogenesis associated with early implantation.

Received August 31, 1998.

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This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Austin, K. J. Articles by Hansen, T. R. Search for Related Content PubMed PubMed Citation Articles by Austin, K. J. Articles by Hansen, T. R.