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Cloning of a 5.8 kb cDNA for a Mouse Type 2 Deiodinase

Department of Physiology, Dartmouth Medical School, Lebana, New Hampshire 03756

Address all correspondence and requests for reprints to: Valeria Anne Galton, Ph.D., Dartmouth Medical School, Department of Physiology, Borwell Building, 1 Medical Center Drive, Lebanon, New Hampshire 03756-0001.

	Abstract

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From studies with their cDNA, the types 1 and 3 deiodinases (D1 and D3) have been shown unequivocally to be selenoproteins. Studies with recently cloned cDNAs for the mammalian type 2 deiodinase (D2) indicate that they also code for selenoproteins. However, these D2 cDNAs are not full length and they do not contain an essential selenocysteine insertion sequence (SECIS) in their 3'UTR; a heterologous SECIS had to be ligated to the coding region before expression of the D2 could be achieved. Thus their role as cDNAs for the native D2 is open to question. We now report the cloning of a 5.8 kb cDNA for the mouse D2. This cDNA contains a SECIS in its 3'UTR located more than 4.5 kb from the coding region. When the mRNA transcribed in vitro from this cDNA is injected into X. laevis oocytes, a deiodinase with characteristics of D2 is expressed.

Received October 9, 1998.

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This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Davey, J. C. Articles by Galton, V. A. Search for Related Content PubMed PubMed Citation Articles by Davey, J. C. Articles by Galton, V. A.