This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pillai, S. B. Articles by Koos, R. D. Search for Related Content PubMed PubMed Citation Articles by Pillai, S. B. Articles by Koos, R. D.

Relaxin Stimulates Uterine Edema via Activation of Estrogen Receptors: Blockade of its Effects Using ICI 182,789, a Specific Estrogen Receptor Antagonist

Department of Physiology (S.B.P., L.C.R., R.D.K.), University of Maryland School of Medicine, Baltimore, Maryland 21201; and Department of Molecular and Integrative Physiology and College of Medicine (O.D.S.), University of Illinois, Urbana, Illinois 61801

Address all correspondence and requests for reprints to Robert D. Koos, Ph.D., University of Maryland Medical School, Department of Physiology, 655 West Baltimore Street, Baltimore, Maryland 21201-1559.

	Abstract

Top Abstract

 
Relaxin’s ability to stimulate uterine growth is well established. The mechanisms by which relaxin exerts this effect, however, remain unclear. In light of previous work demonstrating peptide growth factor activation of estrogen receptors (ERs), the present study was conducted to determine if relaxin similarly stimulates ERs. Twenty-five day-old female Sprague-Dawley rats were bilaterally ovariectomized and treated with estradiol or porcine relaxin alone or in combination with the ER antagonist ICI 182,780. Following treatment with 17ß-estradiol or relaxin alone, the uterine weight/body weight ratio (UtW/BW) increaased significantly over control values (+98% and +77%, respectively, p < 0.0003). Pre-treatment of animals with ICI 182,780 (3 µg/g BW) prior to either estradiol or relaxin treatment completely inhibited the hormone-induced increases in uterine weight (p < 0.0005). ICI 182,780 alone had no significant effect. Histological analysis of uterine cross-sections revealed that the edema present in the endometrium of animals treated with estradiol or relaxin alone was completely absent in the uteri of animals pre-treated with ICI 182,780. These data indicate that relaxin-induced uterine edema and growth is mediated by ERs.

Received January 6, 1999.

This article has been cited by other articles:

				C. Simon and A. Einspanier The hormonal induction of cervical remodeling in the common marmoset monkey (Callithrix jacchus) Reproduction, March 1, 2009; 137(3): 517 - 525. [Abstract] [Full Text] [PDF]

				W. Yan, J. Chen, A. A Wiley, B. D Crean-Harris, F. F Bartol, and C. A Bagnell Relaxin (RLX) and estrogen affect estrogen receptor {alpha}, vascular endothelial growth factor, and RLX receptor expression in the neonatal porcine uterus and cervix Reproduction, May 1, 2008; 135(5): 705 - 712. [Abstract] [Full Text] [PDF]

				A. A. Kazi and R. D. Koos Estrogen-Induced Activation of Hypoxia-Inducible Factor-1{alpha}, Vascular Endothelial Growth Factor Expression, and Edema in the Uterus Are Mediated by the Phosphatidylinositol 3-Kinase/Akt Pathway Endocrinology, May 1, 2007; 148(5): 2363 - 2374. [Abstract] [Full Text] [PDF]

				W. Yan, A. A. Wiley, R. A. D. Bathgate, A.-L. Frankshun, S. Lasano, B. D. Crean, B. G. Steinetz, C. A. Bagnell, and F. F. Bartol Expression of LGR7 and LGR8 by Neonatal Porcine Uterine Tissues and Transmission of Milk-Borne Relaxin into the Neonatal Circulation by Suckling Endocrinology, September 1, 2006; 147(9): 4303 - 4310. [Abstract] [Full Text] [PDF]

				W. Yan, P. L Ryan, F. F Bartol, and C. A Bagnell Uterotrophic effects of relaxin related to age and estrogen receptor activation in neonatal pigs. Reproduction, May 1, 2006; 131(5): 943 - 950. [Abstract] [Full Text] [PDF]

				E.S. Hayes, E.C. Curnow, A.O. Trounson, L.A. Danielson, and E.N. Unemori Implantation and Pregnancy Following In Vitro Fertilization and the Effect of Recombinant Human Relaxin Administration in Macaca fascicularis Biol Reprod, November 1, 2004; 71(5): 1591 - 1597. [Abstract] [Full Text] [PDF]

				O. D. Sherwood Relaxin's Physiological Roles and Other Diverse Actions Endocr. Rev., April 1, 2004; 25(2): 205 - 234. [Abstract] [Full Text] [PDF]

				A. L. Siebel, H. M. Gehring, I. G. T. Reytomas, and L. J. Parry Inhibition of Oxytocin Receptor and Estrogen Receptor-{alpha} Expression, But Not Relaxin Receptors (LGR7), in the Myometrium of Late Pregnant Relaxin Gene Knockout Mice Endocrinology, October 1, 2003; 144(10): 4272 - 4275. [Abstract] [Full Text] [PDF]

				C. Richard, J. Gao, N. Brown, and J. Reese Aquaporin Water Channel Genes Are Differentially Expressed and Regulated by Ovarian Steroids during the Periimplantation Period in the Mouse Endocrinology, April 1, 2003; 144(4): 1533 - 1541. [Abstract] [Full Text] [PDF]

				S. B. Pillai, J. M. Jones, and R. D. Koos Treatment of Rats with 17{beta}-Estradiol or Relaxin Rapidly Inhibits Uterine Estrogen Receptor {beta}1 and {beta}2 Messenger Ribonucleic Acid Levels Biol Reprod, December 1, 2002; 67(6): 1919 - 1926. [Abstract] [Full Text] [PDF]

				C. Binder, Th. Hagemann, B. Husen, M. Schulz, and A. Einspanier Relaxin enhances in-vitro invasiveness of breast cancer cell lines by up-regulation of matrix metalloproteases Mol. Hum. Reprod., September 1, 2002; 8(9): 789 - 796. [Abstract] [Full Text] [PDF]

				S. Hombach-Klonisch, S. Seeger, G. Tscheudschilsuren, J. Buchmann, B. Huppertz, G. Seliger, B. Fischer, and T. Klonisch Cellular localization of human relaxin-like factor in the cyclic endometrium and placenta Mol. Hum. Reprod., April 1, 2001; 7(4): 349 - 356. [Abstract] [Full Text] [PDF]

				Z. S. Kyriakides, P. Petinakis, L. Kaklamanis, E. Sbarouni, P. Karayannakos, D. Iliopoulos, I. Dontas, and D. Th. Kremastinos Intramuscular administration of estrogen may promote angiogenesis and perfusion in a rabbit model of chronic limb ischemia Cardiovasc Res, February 16, 2001; 49(3): 626 - 633. [Abstract] [Full Text] [PDF]

				S. Hombach-Klonisch, M. Abd-Elnaeim, J. A. Skidmore, R. Leiser, B. Fischer, and T. Klonisch Ruminant Relaxin in the Pregnant One-Humped Camel (Camelus dromedarius) Biol Reprod, April 1, 2000; 62(4): 839 - 846. [Abstract] [Full Text]

				L. Zhao, C. S. Samuel, G. W. Tregear, F. Beck, and E. M. Wintour Collagen Studies in Late Pregnant Relaxin Null Mice Biol Reprod, March 1, 2000; 63(3): 697 - 703. [Abstract] [Full Text]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pillai, S. B. Articles by Koos, R. D. Search for Related Content PubMed PubMed Citation Articles by Pillai, S. B. Articles by Koos, R. D.