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Identification of Vitronectin as a Novel Insulin-Like Growth Factor-II Binding Protein

Cooperative Research Centre for Tissue Growth and Repair and CSIRO Human Nutrition (Z.U., H.W., K.H., C.A.Y., G.I.F., F.J.B.), Adelaide BC, SA 5000, Australia; United States Department of Agriculture (J.P.M.), Growth Laboratory, Agricultural Research Service, Beltsville, Maryland 20705

Address all correspondence and requests for reprints to: Zee Upton, Flinders University of South Australia, GPO Box 2100, School of Biology, Adelaide, South Australia, Australia.

	Abstract

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We have previously reported the presence of a 70 kDa insulin-like growth factor (IGF)-II-specific binding protein in chicken serum using Western ligand blotting approaches. In order to ascertain the identity of this 70 kDa IGF-II binding species, the protein has been purified from chicken serum using a combination of ion-exchange and gel-permeation chromatography. Interestingly, amino acid sequencing of the purified protein revealed that it has the same N-terminal sequence as chicken vitronectin (VN). The protein has the ability to specifically bind IGF-II and not IGF-I as determined by ligand blotting, cross-linking and competitive binding assay approaches. In addition, the protein binds ¹²⁵I-des(1-6)-IGF-II, suggesting that the interaction with IGF-II is different to those with other characterized IGF-binding proteins. Importantly, we have ascertained that both human and bovine VN also specifically bind IGF-II. These results are particularly relevant in the light of the recent report that the urokinase-type plasminogen activator receptor, a protein that also binds VN, has been shown to associate with the cation-independent mannose-6-phosphate/IGF-II receptor and suggest a possible role for IGF-II in cell adhesion and invasion.

Received February 17, 1999.

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This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Upton, Z. Articles by Ballard, F. J. Search for Related Content PubMed PubMed Citation Articles by Upton, Z. Articles by Ballard, F. J.