This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rushing, P. A. Articles by Woods, S. C. Search for Related Content PubMed PubMed Citation Articles by Rushing, P. A. Articles by Woods, S. C.

Amylin: A Novel Action in the Brain to Reduce Body Weight*

Department of Psychiatry (P.A.R., M.M.H., R.J.S., S.C.W.) College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0559; and Institute of Veterinary Physiology (T.A.L.), University of Zürich, CH 8057 Zürich, Switzerland

Address all correspondence and requests for reprints to: Paul A. Rushing, Ph.D., University of Cincinnati, College of Medicine, Department of Psychiatry, Cincinnati, Ohio 45267-0559.

	Abstract

Top Abstract

 
Amylin is a 37-amino acid peptide hormone that is co-secreted with insulin by pancreatic B cells in response to a nutrient stimulus (e.g., during meals). To test the hypothesis that amylin acts within the brain to reduce long-term food intake and body weight, we examined the effects of acute and chronic 3^rd-ventricular (i3vt) infusion of low doses of amylin on food intake and body weight in rats.

In one experiment, separate groups of ad lib-fed male Long Evans rats were given one i3vt infusion (3 µl over 30 s) of synthetic cerebrospinal fluid vehicle or 1 to 100 pmol amylin, and food intake and body weight were monitored for 7 days. Amylin potently and dose-dependently reduced 1-h food intake, with all doses producing significant reductions. The largest dose (100 pmol) significantly reduced 24-h intake by over 30%. The effect was persistent in that both 7-day cumulative food intake and body weight change were significantly decreased over the 7 days following a single injection of 100 pmol of amylin.

Other groups of rats received continuous i3vt infusion (0.5 µl/h volume) of saline or 2.0 pmol/h amylin via osmotic minipumps over 10 days. Food intake over the 10-day infusion was significantly suppressed in amylin-treated rats as compared to that of controls. Consequently, by the 4^th day of infusion, amylin rats weighed significantly less than baseline relative to saline controls, and this difference persisted throughout the remainder of the infusion period. At sacrifice (Day 10), the percent of body weight from retroperitoneal fat depots was significantly lower in the amylin-treated rats, indicative of a reduction of total body adiposity.

In summary, the results support the hypothesis that amylin acts as a signal to the brain contributing to the maintenance of long-term energy balance.

Received October 7, 1999.

This article has been cited by other articles:

				S. R. Bloom, F. P. Kuhajda, I. Laher, X. Pi-Sunyer, G. V. Ronnett, T. M.M. Tan, and D. S. Weigle The Obesity Epidemic: Pharmacological Challenges Mol. Interv., April 1, 2008; 8(2): 82 - 98. [Abstract] [Full Text] [PDF]

				C. J. Price, T. D. Hoyda, and A. V. Ferguson The Area Postrema: A Brain Monitor and Integrator of Systemic Autonomic State Neuroscientist, April 1, 2008; 14(2): 182 - 194. [Abstract] [PDF]

				J. D. Roth, T. Coffey, C. M. Jodka, H. Maier, J. R. Athanacio, C. M. Mack, C. Weyer, and D. G. Parkes Combination Therapy with Amylin and Peptide YY[3 36] in Obese Rodents: Anorexigenic Synergy and Weight Loss Additivity Endocrinology, December 1, 2007; 148(12): 6054 - 6061. [Abstract] [Full Text] [PDF]

				P. K. Chelikani, A. C. Haver, and R. D. Reidelberger Effects of intermittent intraperitoneal infusion of salmon calcitonin on food intake and adiposity in obese rats Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R1798 - R1808. [Abstract] [Full Text] [PDF]

				J. D. Roth, H. Hughes, T. Coffey, H. Maier, J. L. Trevaskis, and C. M. Anderson Effects of prior or concurrent food restriction on amylin-induced changes in body weight and body composition in high-fat-fed female rats Am J Physiol Endocrinol Metab, October 1, 2007; 293(4): E1112 - E1117. [Abstract] [Full Text] [PDF]

				M. Fry, T. D. Hoyda, and A. V. Ferguson Making Sense of It: Roles of the Sensory Circumventricular Organs in Feeding and Regulation of Energy Homeostasis Experimental Biology and Medicine, January 1, 2007; 232(1): 14 - 26. [Abstract] [Full Text] [PDF]

				J. D. Roth, H. Hughes, E. Kendall, A. D. Baron, and C. M. Anderson Antiobesity Effects of the {beta}-Cell Hormone Amylin in Diet-Induced Obese Rats: Effects on Food Intake, Body Weight, Composition, Energy Expenditure, and Gene Expression Endocrinology, December 1, 2006; 147(12): 5855 - 5864. [Abstract] [Full Text] [PDF]

				R. Hood, V. Valentine, S. Mac, and W. H Polonsky Use of Exenatide in Patients With Type 2 Diabetes Diabetes Spectr, July 1, 2006; 19(3): 181 - 186. [Abstract] [Full Text] [PDF]

				K. D. Reynolds and D. Spruijt-Metz Translational research in childhood obesity prevention. Eval Health Prof, June 1, 2006; 29(2): 219 - 245. [Abstract] [PDF]

				D. F. Kruger, C. L. Martin, and C. E. Sadler New insights into glucose regulation. The Diabetes Educator, March 1, 2006; 32(2): 221 - 228. [Abstract] [Full Text] [PDF]

				K. J. Pulman, W. M. Fry, G. T. Cottrell, and A. V. Ferguson The Subfornical Organ: A Central Target for Circulating Feeding Signals J. Neurosci., February 15, 2006; 26(7): 2022 - 2030. [Abstract] [Full Text] [PDF]

				M. C. Riddle and D. J. Drucker Emerging Therapies Mimicking the Effects of Amylin and Glucagon-Like Peptide 1 Diabetes Care, February 1, 2006; 29(2): 435 - 449. [Full Text] [PDF]

				R. D. Reidelberger, A. C. Haver, U. Arnelo, D. D. Smith, C. S. Schaffert, and J. Permert Amylin receptor blockade stimulates food intake in rats Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2004; 287(3): R568 - R574. [Abstract] [Full Text] [PDF]

				S. L. Aronoff, K. Berkowitz, B. Shreiner, and L. Want Glucose Metabolism and Regulation: Beyond Insulin and Glucagon Diabetes Spectr, July 1, 2004; 17(3): 183 - 190. [Abstract] [Full Text] [PDF]

				E. L Kleppinger and E. M Vivian Pramlintide for the Treatment of Diabetes Mellitus Ann. Pharmacother., July 1, 2003; 37(7): 1082 - 1089. [Abstract] [Full Text] [PDF]

				B. A. Baldo and A. E. Kelley Amylin infusion into rat nucleus accumbens potently depresses motor activity and ingestive behavior Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2001; 281(4): R1232 - R1242. [Abstract] [Full Text] [PDF]

				T. A. Lutz, S. Tschudy, A. Mollet, N. Geary, and E. Scharrer Dopamine D2 receptors mediate amylin's acute satiety effect Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1697 - R1703. [Abstract] [Full Text] [PDF]

				J.-M. Ye, M. Lim-Fraser, G. J. Cooney, G. J. S. Cooper, M. A. Iglesias, D. G. Watson, B. Choong, and E. W. Kraegen Evidence that amylin stimulates lipolysis in vivo: a possible mediator of induced insulin resistance Am J Physiol Endocrinol Metab, April 1, 2001; 280(4): E562 - E569. [Abstract] [Full Text] [PDF]

				R. D. Reidelberger, U. Arnelo, L. Granqvist, and J. Permert Comparative effects of amylin and cholecystokinin on food intake and gastric emptying in rats Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2001; 280(3): R605 - R611. [Abstract] [Full Text] [PDF]

				R. D. Reidelberger, L. Kelsey, and D. Heimann Effects of amylin-related peptides on food intake, meal patterns, and gastric emptying in rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2002; 282(5): R1395 - R1404. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rushing, P. A. Articles by Woods, S. C. Search for Related Content PubMed PubMed Citation Articles by Rushing, P. A. Articles by Woods, S. C.