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View larger version (122K): [in this window] [in a new window]   Figure 7. Expression of the transgene and the endogenous gene in male mice of different ages. Adrenal sections from line 78 mice at 3 and 5 weeks of age were stained for ß-galactosidase activity (A and C) or hybridized with CYP11A1 (SSC; B and D) riboprobe. The adrenal X zone had disappeared by 5 weeks of age. The LacZgene expression pattern was similar to SCC gene expression in the X zone and other adrenocortical zones. F, Zona fasciculata; X, X zone; M, medulla.

View larger version (158K): [in this window] [in a new window]   Figure 8. Expression of the transgene and the endogenous gene in female mice of different ages. Adrenal sections from female transgenic mice (line 78) at 3, 7, and 9 weeks of age were stained for ß-galactosidase activity (A, C, and E) or hybridized with the CYP11A1 (SCC; B and D) or LacZ (F) riboprobe. The adrenal X zone started to regress at 9 weeks. SCC and LacZ gene expression in the X zone follows the same sequence. F, Zona fasciculata; X, X zone; M, medulla.

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