This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Juréus, A. Articles by Steiner, R. A. Search for Related Content PubMed PubMed Citation Articles by Juréus, A. Articles by Steiner, R. A.

Galanin-Like Peptide (GALP) Is a Target for Regulation by Leptin in the Hypothalamus of the Rat

Departments of Physiology and Biophysics (A.J., R.A.S.), Obstetrics and Gynecology (D.K.C., R.A.S.), Zoology (R.A.S.), and Graduate Program in Neurobiology and Behavior (M.J.C., M.E.M.), University of Washington, Seattle, Washington 98195-7290

Address all correspondence and requests for reprints to: Robert A. Steiner, Ph.D., University of Washington, Department of Physiology and Biophysics, G-424 Health Sciences Building, Box 357290, Seattle, Washington 98195-8712.

	Abstract

Top Abstract Introduction

 
Galanin-like peptide (GALP), which was recently isolated from the porcine hypothalamus, shares sequence homology with galanin and binds with high affinity to galanin receptors. To study the distribution and regulation of GALP-expressing cells in the brain, we cloned a 120 base-pair cDNA fragment of rat GALP and produced an antisense riboprobe. In situ hybridization for GALP mRNA was then performed on tissue sections throughout the forebrain of adult ovariectomized female rats. We found GALP mRNA-containing cells in the arcuate nucleus (Arc), caudal dorsomedial nucleus, median eminence and the pituitary. Because GALP mRNA in the Arc appeared to overlap with the known distribution of leptin receptor mRNA, we tested the hypothesis that GALP expression is regulated by leptin. Using in situ hybridization, we compared the number of GALP mRNA-containing cells among groups of rats that were fed ad lib or fasted for 48 h and treated with either leptin or vehicle. Fasting reduced the number of identifiable cells containing GALP mRNA in the Arc, whereas the treatment of fasted animals with leptin produced a 4-fold increase in the number of cells expressing GALP message. The presence of GALP mRNA in the hypothalamus and pituitary and its regulation by leptin suggests that GALP may have important neuroendocrine functions, including the physiological regulation of feeding, metabolism, and reproduction.

	Introduction

Top Abstract Introduction

 

Received May 8, 2000.

This article has been cited by other articles:

				B. J Todd, G. S Fraley, A. C Peck, G. J Schwartz, and A. M Etgen Central Insulin-Like Growth Factor 1 Receptors Play Distinct Roles in the Control of Reproduction, Food Intake, and Body Weight in Female Rats Biol Reprod, September 1, 2007; 77(3): 492 - 503. [Abstract] [Full Text] [PDF]

				J. M. Castellano, V. M. Navarro, R. Fernandez-Fernandez, J. Roa, E. Vigo, R. Pineda, R. A. Steiner, E. Aguilar, L. Pinilla, and M. Tena-Sempere Effects of galanin-like peptide on luteinizing hormone secretion in the rat: sexually dimorphic responses and enhanced sensitivity at male puberty Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1281 - E1289. [Abstract] [Full Text] [PDF]

				M. Kuramochi, T. Onaka, D. Kohno, S. Kato, and T. Yada Galanin-Like Peptide Stimulates Food Intake via Activation of Neuropeptide Y Neurons in the Hypothalamic Dorsomedial Nucleus of the Rat Endocrinology, April 1, 2006; 147(4): 1744 - 1752. [Abstract] [Full Text] [PDF]

				A. G. Stoyanovitch, M. A. Johnson, D. K. Clifton, R. A. Steiner, and G. S. Fraley Galanin-Like Peptide Rescues Reproductive Function in the Diabetic Rat Diabetes, August 1, 2005; 54(8): 2471 - 2476. [Abstract] [Full Text] [PDF]

				G. S. Fraley, J. M. Scarlett, I. Shimada, D. N. Teklemichael, B. V. Acohido, D. K. Clifton, and R. A. Steiner Effects of Diabetes and Insulin on the Expression of Galanin-Like Peptide in the Hypothalamus of the Rat Diabetes, May 1, 2004; 53(5): 1237 - 1242. [Abstract] [Full Text] [PDF]

				M. J. Cunningham, M. Shahab, K. L. Grove, J. M. Scarlett, T. M. Plant, J. L. Cameron, M. S. Smith, D. K. Clifton, and R. A. Steiner Galanin-Like Peptide as a Possible Link between Metabolism and Reproduction in the Macaque J. Clin. Endocrinol. Metab., April 1, 2004; 89(4): 1760 - 1766. [Abstract] [Full Text] [PDF]

				A. Seth, S. Stanley, P. Jethwa, J. Gardiner, M. Ghatei, and S. Bloom Galanin-Like Peptide Stimulates the Release of Gonadotropin-Releasing Hormone in Vitro and May Mediate the Effects of Leptin on the Hypothalamo-Pituitary-Gonadal Axis Endocrinology, February 1, 2004; 145(2): 743 - 750. [Abstract] [Full Text] [PDF]

				M. G. Myers Jr. Leptin Receptor Signaling and the Regulation of Mammalian Physiology Recent Prog. Horm. Res., January 1, 2004; 59(1): 287 - 304. [Abstract] [Full Text]

				M. L. Heiman and M. A. Statnick Galanin-Like Peptide Functions More Like Leptin than Like Galanin Endocrinology, November 1, 2003; 144(11): 4707 - 4708. [Full Text] [PDF]

				K. R. Hansen, S. M. Krasnow, M. A. Nolan, G. S. Fraley, J. W. Baumgartner, D. K. Clifton, and R. A. Steiner Activation of the Sympathetic Nervous System by Galanin-Like Peptide--A Possible Link between Leptin and Metabolism Endocrinology, November 1, 2003; 144(11): 4709 - 4717. [Abstract] [Full Text] [PDF]

				C. B. Lawrence, T. Williams, and S. M. Luckman Intracerebroventricular Galanin-Like Peptide Induces Different Brain Activation Compared with Galanin Endocrinology, September 1, 2003; 144(9): 3977 - 3984. [Abstract] [Full Text] [PDF]

				S. Kumano, H. Matsumoto, Y. Takatsu, J. Noguchi, C. Kitada, and T. Ohtaki Changes in Hypothalamic Expression Levels of Galanin-Like Peptide in Rat and Mouse Models Support That It Is a Leptin-Target Peptide Endocrinology, June 1, 2003; 144(6): 2634 - 2643. [Abstract] [Full Text] [PDF]

				G. S. Fraley, I. Shimada, J. W. Baumgartner, D. K. Clifton, and R. A. Steiner Differential Patterns of Fos Induction in the Hypothalamus of the Rat Following Central Injections of Galanin-Like Peptide and Galanin Endocrinology, April 1, 2003; 144(4): 1143 - 1146. [Abstract] [Full Text] [PDF]

				S. M. Krasnow, G. S. Fraley, S. M. Schuh, J. W. Baumgartner, D. K. Clifton, and R. A. Steiner A Role for Galanin-Like Peptide in the Integration of Feeding, Body Weight Regulation, and Reproduction in the Mouse Endocrinology, March 1, 2003; 144(3): 813 - 822. [Abstract] [Full Text] [PDF]

				M. J. Cunningham, J. M. Scarlett, and R. A. Steiner Cloning and Distribution of Galanin-Like Peptide mRNA in the Hypothalamus and Pituitary of the Macaque Endocrinology, March 1, 2002; 143(3): 755 - 763. [Abstract] [Full Text] [PDF]

				A. Jureus, M. J. Cunningham, D. Li, L. L. Johnson, S. M. Krasnow, D. N. Teklemichael, D. K. Clifton, and R. A. Steiner Distribution and Regulation of Galanin-Like Peptide (GALP) in the Hypothalamus of the Mouse Endocrinology, December 1, 2001; 142(12): 5140 - 5144. [Abstract] [Full Text] [PDF]

				Y. Takatsu, H. Matsumoto, T. Ohtaki, S. Kumano, C. Kitada, H. Onda, O. Nishimura, and M. Fujino Distribution of Galanin-Like Peptide in the Rat Brain Endocrinology, April 1, 2001; 142(4): 1626 - 1634. [Abstract] [Full Text]

This Article Abstract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Juréus, A. Articles by Steiner, R. A. Search for Related Content PubMed PubMed Citation Articles by Juréus, A. Articles by Steiner, R. A.