Figure 7. Regulation of p27 mRNA by IFNs and ER in Ishikawa
endometrial cells. A, Induction of p27 mRNA in response to different
types of IFNs in Ishikawa cells was analyzed by RT-PCR. Lanes v, 
,
, and ß, Cells treated with vehicle or 1000 U/ml IFN, IFN,
and IFNß, respectively. B, Upper panel, Northern blot
analysis of p27 mRNA in Ishikawa cells. Cells were incubated with
solvent (lane 1), 10-7 M estrogen
(lane 2), 10-7 M estrogen plus
10-5 M ICI 182,780 (lane 3), 1000
U/ml IFN (lane 4), and 1000 U/ml f IFN plus
10-7 M estrogen (lane 5).
Lower panel, Quantitation of p27 RNA signals was
performed by densitometric scanning of the bands in the Northern blot
shown in the above panel followed by normalization with respect to
GAPDH signals (not shown).
