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Erratum

In the article by H. Hui et al. (Endocrinology 143:3529–3539, 2002), Figs. 8 and 10 were incorrectly numbered. What appeared as Fig. 8 is actually Fig. 10 and what appeared as Fig. 10 is actually Fig. 8. The correctly numbered figures, as well as their respective legends, appear below.

View larger version (26K): [in this window] [in a new window]   Figure 8. GLP-1R expression in MIN-6 cells transfected with RIP/GLP-1. Parental, CVM/GLP-1-transfected, and RIP/GLP-1 transfected MIN-6 cells were cultured for 12 h in the presence of 6 mM glucose. After removal of the culture medium, the cells were collected and the protein extract subjected to Western blot analysis with a polyclonal antibody directed against human GLP-1R. A, An individual Western blot analysis. B, A graph representing the average of three independent experiments, with the GLP-1R levels normalized by the total protein content of each individual cell extract. For glucose response study, MIN-6 cells transfected with RIP/GLP-1 were cultured in serum-free medium in the presence of various concentrations of glucose (0, 3, 6, 15 mM) for 12 h. C, An individual Western blot analysis. D, A graph representing the average of three independent experiments, with the GLP-1R levels normalized by the total protein content of each individual cell extract.

View larger version (25K): [in this window] [in a new window]   Figure 10. Effect of cAMP inhibition on glucose and GLP-1-dependent secretion of insulin and insulin mRNA levels. MIN-6 RIP/GLP-1 cells routinely cultured in the presence of 10% FBS and 12 mM glucose were subjected to an overnight washout period with medium deprived of glucose and FBS. They were then cultured in serum-free medium in the presence of either 3 mM or 10 mM glucose in the presence of Rp-cAMP (10-6 M). A, The cAMP levels normalized for protein content. B, Insulin levels normalized for protein content. C, The mRNA levels for insulin, normalized for ß-actin mRNA content. The blot in C indicates one individual experiment; repetition of the experiment using RNA extracts from independent cultures produced very similar results. Statistical significance of the data for mRNA and protein levels was evaluated by ANOVA. P values indicated in the figure were derived by comparing the two curves obtained from cells treated with Rp-cAMP vs. control.

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