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Correction for Hui et al., Endocrinology 143 (9) 3529-3539.
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Endocrinology Vol. 143, No. 11 4349
Copyright © 2002 by The Endocrine Society

ANNOUNCEMENT

Erratum

In the article by H. Hui et al. (Endocrinology 143:3529–3539, 2002), Figs. 8Go and 10Go were incorrectly numbered. What appeared as Fig. 8Go is actually Fig. 10Go and what appeared as Fig. 10Go is actually Fig. 8Go. The correctly numbered figures, as well as their respective legends, appear below.



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  		Figure 8. GLP-1R expression in MIN-6 cells transfected with RIP/GLP-1. Parental, CVM/GLP-1-transfected, and RIP/GLP-1 transfected MIN-6 cells were cultured for 12 h in the presence of 6 mM glucose. After removal of the culture medium, the cells were collected and the protein extract subjected to Western blot analysis with a polyclonal antibody directed against human GLP-1R. A, An individual Western blot analysis. B, A graph representing the average of three independent experiments, with the GLP-1R levels normalized by the total protein content of each individual cell extract. For glucose response study, MIN-6 cells transfected with RIP/GLP-1 were cultured in serum-free medium in the presence of various concentrations of glucose (0, 3, 6, 15 mM) for 12 h. C, An individual Western blot analysis. D, A graph representing the average of three independent experiments, with the GLP-1R levels normalized by the total protein content of each individual cell extract.

 


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  		Figure 10. Effect of cAMP inhibition on glucose and GLP-1-dependent secretion of insulin and insulin mRNA levels. MIN-6 RIP/GLP-1 cells routinely cultured in the presence of 10% FBS and 12 mM glucose were subjected to an overnight washout period with medium deprived of glucose and FBS. They were then cultured in serum-free medium in the presence of either 3 mM or 10 mM glucose in the presence of Rp-cAMP (10-6 M). A, The cAMP levels normalized for protein content. B, Insulin levels normalized for protein content. C, The mRNA levels for insulin, normalized for ß-actin mRNA content. The blot in C indicates one individual experiment; repetition of the experiment using RNA extracts from independent cultures produced very similar results. Statistical significance of the data for mRNA and protein levels was evaluated by ANOVA. P values indicated in the figure were derived by comparing the two curves obtained from cells treated with Rp-cAMP vs. control.

 





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