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FIG. 1. Migration of MCF-7 cells through Transwells in response to IGF-I prebound to VN in the absence or presence of IGFBP-5. MCF-7 cells were seeded onto Transwells that had been coated with VN ± IGF-I ± IGFBP-5 and allowed to migrate through the porous membrane for 5 h. The number of cells transversing the membrane in the presence of IGF-I were then expressed as a percentage of those that migrated on VN alone. The responses are shown as IGF-I exposed to VN in the absence of IGFBP-5 ( ), the presence of IGFBP-5 (
), and the response of IGFBP-5 alone ( ). Values shown are the mean ± SEM of duplicate wells from three experiments. *, P < 0.05.
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