This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content

Erratum

In the article "Vascular Lipotoxicity: Endothelial Dysfunction via Fatty-Acid-Induced Reactive Oxygen Species Overproduction in Obese Zucker Diabetic Fatty Rats" by Ichiro Chinen, Michio Shimabukuro, Ken Yamakawa, Namio Higa, Toshihiro Matsuzaki, Katsuhiko Noguchi, Shinichiro Ueda, Matao Sakanashi, and Nobuyuki Takasu (Endocrinology 148:160–165), the authors note two errors. On page 161, the entire paragraph under "Immunoprecipitations and Western Blotting" is incorrect. The correct paragraph is as follows:

Western blot analysis was performed as described previously (15). Protein samples (200 µg) were prepared from thoracic aortas of four groups of rats and incubated with anti-eNOS (Santa Cruz Biotechnology, Santa Cruz, CA) or anti-p47phox (Upstate, Charlottesville, VA) antibodies at 4 C for 16 h on a rocking platform. Fifty microliters of protein G-agarose suspension (Roche, Germany) was then added and incubated for 2 h at 4 C. After washing the beads, according to manufacturer’s manual, the samples were centrifuged at 12,000 x g for 20 sec, and were subjected to SDS-PAGE. After transfer onto polyvinylidene difluoride transfer membranes, the membranes were probed with anti-phosphoserine antibody (Chemicon International, Temecula, CA), and immunoreactive proteins were detected by chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate; Pierce, Rockford, IL). Band intensity was quantified by NIH ImageJ 1.32j, and the ratio of anti-phosphoserine blot intensity to those of eNOS or p47phox blot intensity was used to represent the enzyme catalytic activities. Lipid peroxidation level of aorta was determined using the TBARS assay kit (ZeptoMetrix Corp., Buffalo, NY).

On page 163, above the left panel in Fig. 2, the text "tyrosine phosphorylation of eNOS" should read "serine phosphorylation of eNOS."

The authors regret and apologize for any problems these errors may have caused.

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content