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FIG. 7. Effect of NF- B on TWEAK-induced inhibition of insulin action. A, Insulin-induced Akt phosphorylation was assessed in Huh7 cells after the indicated treatment in the presence or absence of Bay 11-7082 (5 µM), MG132 (0.5 µM), or vehicle alone; B, Huh7 cells were transfected with p65 or scrambled control siRNAs. After 36 h, cells were pretreated for 2 h with TWEAK followed by insulin (10 nmol/liter) stimulation, and cell lysates were subjected to Western blot assays using antibodies against phospho-Akt (pAkt) (Ser 473), total Akt, or p65. The histograms represent band intensities that were normalized to total Akt levels. Data are expressed as mean ± SD derived from three separate experiments. *, P < 0.01, Bay 11-7082 or MG132-treated vs. vehicle alone (A); *, P < 0.01, p65 siRNA transfected cells vs. the control (B).
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