Deletion of the Nhlh2 Transcription Factor Decreases the Levels of the Anorexigenic Peptides Melanocyte-Stimulating Hormone and Thyrotropin-Releasing Hormone and Implicates Prohormone Convertases I and II in Obesity

Supplemental Data

Supplemental figures and table.

Files in this Data Supplement:

• Supplemental Figure 1 - N2KO mice have reduced levels of PC2 mRNA in their ARC, but not in the PVN and LH. (A-F) In situ hybridization using a cRNA probe to murine PC2 on coronal section of hypothalamus from normal (A, C, E) and N2KO (B, D, F) mice. (A-B) ARC, 20X magnification (C-D) PVN, 20X magnification (E-F) LH, 40X magnification.
• Supplemental Figure 2 - Quantification of PC2 mRNA shows reduced levels of mRNA only in the ARC of N2KO mice. Comparison of PC2 mRNA signal intensity (measured as pixel counts) in WT and N2KO mice in the ARC (P < 0.05, Student’s t test), PVN and LH. Data are represented as mean + SEM.
• Supplemental Table 1