Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Table S1 - Primers for PCR Procedures
• Supplemental Figure S1 - DNA methylation analysis of F3 generation control (Con) and vinclozolin (Tre) caudal epididymal sperm. Gel images of PCR based methylation sensitive restriction analysis screen. All candidates listed on right margin.
• Supplemental Figure S2 - Summary of altered methylation changes by bisulfite sequencing analysis for each confirmed candidate. Shaded box are statistically (p<0.05) different changes with all methylation percentages for each site presented.
• Supplemental Figure S3 - Methylation states of candidates with bisulfite sequencing with the physical chromosome map with alignment (arrow), DNA sequence of interest, and bisulfite results presented.
• Supplemental Figure S4 - Pathway assist analysis of gene interactions with the candidates(open) and interacting genes(shaded) with cellular pathways (rectangles).