Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Immunocyochemical localizations of mitochondrial proteins associated with apoptosis and caspases in RL95-2 endometrial cancer cells after exposure to benzo[a]pyrene (B[a]P). The RL95-2 cells were treated with B[a]P (10 μM) for 96 h. The nuclei were counterstained with propidium iodide (red). Con and B[a]P indicate control and benzo[a]pyrene, respectively. Microphotographs were taken using confocal microscopy. Original magnification: x 800. A, Localizations of cytochrome c, Smac/DIABLO, and AIF. B: Localizations of active caspase-8, -9, and -3
• Supplemental Figure 2 - Immunolocalization of AhR and Hsp90. Cells were treated for 48 h with 10 μM benzo[a]pyrene (B[a]P), cytospun, fixed, and immunostained with antibodies as indicated. The nuclei were counterstained with propidium iodide (red). The images were taken by a confocal microscope. a, Control: AhR; b, B[a]P-treated: AhR; c, control: Hsp90; d, B[a]P-treated: Hsp90. Original magnification: x 800.
• Supplemental Figure 3 - Immunolocalization of CYP1A1, CYP1B1, and EH. Cells were treated for 48 h with 10 μM benzoapyrene (BaP), cytospun, fixed, and immunostained with antibodies as indicated. The nuclei were counterstained with propidium iodide (red). The images were taken by a confocal microscope. a, Control: CYP1A1; b, BaP-treated: CYP1A1; c, control: CYP1B1; d, BaP-treated: CYP1B1: e, Control: EH; f, BaP-treated: EH. Original magnification: x 800.