Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Table 1 - Effect of calcium chelators and signaling inhibitors on viability (%) of bovine granulosa cells on day 7 of serum-free culture
• Supplemental Figure 1 - A: Representative profiles of the Ca²⁺ responses induced by ionophore (A23187; 1 μM) and FSH (25 ng /ml). The arrow represents the time point of A23187 and FSH stimulation. Granulosa cells were isolated and prepared for fura-2 AM measurement of [Ca²⁺]_i as described in Materials and Methods. Data are depicted as the relative fluorescence ratio (340:380 nm) over time (in seconds).

  B: Representative profiles of the Ca²⁺ responses induced by FSH (25 ng/ml) in the presence or absence of CART peptide (0.1 μM) in Ca²⁺ free media. The arrow represents the time point of FSH stimulation. Granulosa cells were isolated and prepared for fura-2 AM measurement of [Ca²⁺]_i in Ca²⁺ free media as described in Materials and Methods. Data are depicted as the relative fluorescence ratio (340:380 nm) over time (in seconds).