Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Effect of chronic hormone imbalance and high-fat diet (HFD) on deep visceral adipose tissue Representative images of mesenteric arterial branch, covered by adipose tissue in the 4 groups of mice examined at 9 months are shown. Such images were used to compute width measurements shown in Text Fig 1B.
• Supplemental Figure 2 - Determination of changes in HMW forms of adiponectin. A: Representative blot of plasma HMW adiponectin forms at 9-months in the 4 groups assessed by using non-reducing and non-heat-denaturing SDS-PAGE as well as total adiponectin expression in the same sample by using reducing conditions. Adiponectin was detected using anti-adiponectin antibody. B: Nature of changes during aging in WT and FORKO mice. A representative blot of plasma HMW adiponectin expression at 3, 5, 9-month in WT and FORKO female mice is shown.