Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - PPT and DPN mimic the effect of E2 in preventing the OVX-induced increase in immobility in the forced swim test (FST). Hormone treatments performed in 3xTg-AD were replicated in wild-type female C57Bl6/129S mice. In brief, wild-type mice at age 3 mo (N = 6-7 per group) were bilaterally ovariectomized (OVX) or sham OVX, then immediately treated with either placebo (Sham OVX, OVX groups), E2 (OVX+E2), PPT (OVX+PPT), or DPN (OVX+DPN). Two weeks following hormone manipulations, animals were evaluated for 5 minutes on the FST and scored for immobility at every 5-second interval during minutes 2-5. Data show mean percentage of time spent immobile. In comparison to Sham OVX group, the OVX group exhibited significantly higher immobility. The OVX+E2, OVX+PPT and OVX+DPN groups showed immobility values that were significantly less than the OVX group but not significantly different from the Sham OVX group F (4,22) = 2.96, P = 0.04. * Denotes P < 0.05 compared to Sham OVX, # P < 0.05 compared to OVX