Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - a- Representative photomicrograph showing tryptophan hydroxylase immunopositive cells in the VMN of C. uniparens. Cells were visualized using a polyclonal sheep anti-tryptophan hydroxylase antibody. b, c- Representative autoradiogaphs illustrating 5-HT_1A and 5-HT_2A receptor mRNA in the brain of C. uniparens. Left half shows labeling of antisense probe using in situ hybridization, and right half shows labeling with sense probe as a control.
• Supplemental Figure 2 - Data represented as number of tryptophan hydroxylase immunopositive cells x 10 ⁹ ± SEM. A univariate ANOVA was used to analyze differences in TrpH-immunoreactive cells numbers in the POA and VMN of with hormonal manipulation (preovulatory vs postovulatory, and OVX+E vs OVX+T as independent analyses) and region as the independent variables, and cell number as the dependent variable. No significant differences were observed between any of the groups examined.
• Supplementary Video 1 - Supplementary Video 1: Lizard Pseudopopulation (Short Version)