Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplementary Figure 1 - GnRHR-TAg transgenic mouse genotyping and gonadal phenotype. A) Representative image of agarose gel separation of PCR products from genotyping of littermates from the GnRHR-TAg M29 transgenic mouse line. M, DNA size ladder; NT, nontransgenic; T, transgenic; H₂O (Ctrl), water control; upper arrowhead, GnRHR-TAg PCR product (892 bp); lower arrowhead, GnRHR PCR product (736 bp). B) Representative images of nontransgenic and transgenic female (top) and male (bottom) internal reproductive organs from 2 and 5 month-old littermates. GnRHR-TAg transgenic female mice have underdeveloped internal reproductive organs. NT, nontransgenic; T, transgenic; bars = 3mm.
• Supplementary Figure 2 - GnRHR-TAg transgenic female mice express LHβ and FSHβ in distinct pituitary cell populations. Immuno-colocalization of LHβ and FSHβ in female (A) and male (B) pituitaries at 2 or 5 months of age. Below the merged images are two inserts of showing images of LHβ (green; lower left) and FSHβ (red; lower right) immunostaining. 2M, 2 months of age; 5M, 5 months of age; NT, nontransgenic; T, GnRHR-TAg transgenic; magnification, 400x; bars = 20 μm.
• Supplementary Figure 3 - IRMA analysis of serum gonadotropin levels in GnRHR-TAg transgenic mice. Plasma samples were analyzed for FSH (A) and LH (B) by IRMA in 5 month old female and male mice. NT, nontransgenic; T, transgenic; *, P<0.05 vs. nontransgenic littermate controls at the same age.
• Supplementary Table 1 - Genotyping Primers and Quantitative PCR Primers