Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure S1 - BrdU immuno-staining of microadenomas that formed in a 4 month-old, SST-/-,Tg pituitary.
• Supplemental Figure S2 - (A) Selected section of the membrane array showing 3 genes (GSTM2,NME2 and TNFR2) that were differentially expressed in adenomatous vs. hyperplastic pituitaries. Specifically the top panels are from an array hybridized with a hyperplastic cDNA probe (generated from pooled RNA of 2 hyperplastic pituitaries weighing < 7 mg each) while the bottom panels are from an array hybridized with a tumor cDNA probe (generated from a single pituitary containing multiple raised nodules weighing >100mg). (B) Confirmation of differential expression, by qrtRT-PCR, of genes found by membrane array to be the most altered in hyperplastic vs. WT pituitaries and in adenomatous Tg vs. hyperplastic Tg pituitaries, as shown in Table 1 in the main text. cDNA samples used for qrtRT-PCR were the same as those used to evaluate Ki67, men1, p27 and SST receptor expression in male WT and Tg mice (Figs 1A, 1D and Fig. 3). a, represents values that differ from age-matched WT controls (p<0.05) and asterisks (*, p<0.05; **, p<0.01), indicates values that significantly differ between age within genotype.
• Supplemental Table S1 - Mouse-specific primers used for quantitative real-time, RT-PCR
• Supplemental Table S2 - Absolute cDNA copy number / 0.05 μg total RNA of gene transcripts in the pituitary of 4- and ≥ 10-month old WT and Tg male mice, as determined by quantitative real-time RT-PCR.