Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure S1 - H₂O₂-scavenging activity in isolated islets of B6/Ucp2-/- mice. n = 3 for each genotype. *, p < 0.05 vs. Ucp2+/+.
• Supplemental Figure S2 - A) B6/Ucp2-/- islets exhibit lower insulin secretion in response to glucose and diethyl maleate (DEM) challenges. *, p < 0.05 vs. 3 mM glucose alone of the same genotype; #, p < 0.05 vs. Ucp2+/+ with the same treatment.
  B) A/J/Ucp2-/- islets exhibit lower insulin secretion in response to glucose and diethyl maleate (DEM) challenges. *, p < 0.05 vs. 3 mM glucose alone of the same genotype; #, p < 0.05 vs. Ucp2+/+ with the same treatment.
• Supplemental Figure S3 - 129/Ucp2-/- islets exhibit lower insulin secretion in response to glucose and KCl challenges. *, p < 0.05 vs. 3 mM glucose alone of the same genotype; #, p < 0.05 vs. Ucp2+/+ with the same treatment.
• Supplemental Figure S4 - Glucose tolerance test in Ucp2+/+ and Ucp2-/- mice on different genetic backgrounds. Fasted mice received glucose (1.5 g/kg body weight) by i.p. Blood samples collected from tail bleeds were immediately analyzed for glucose using FreeStyle Blood Glucose Monitoring System (TheraSense, Inc. Alameda, CA). A: B6 (N20, 4 month old). n = 4 for each genotype; B: A/J (N16, 5-6 month old). n = 6 for each genotype; C: Jax B6 (these are 129.B9 Ucp2^tm1Lowl; N10, 4 months old). N = 5 for each genotype; D: 129 (N18, 4 month old). Ucp2+/+, n = 4; Ucp2-/-, n = 5. Two-way ANOVA with Bonferroni post hoc testing indicates no significant difference between Ucp2+/+ and -/-, except for the 30 minute time-point in the Jax B6.
• Supplemental Figure S5 - Insulin content (A) and GSIS (B) in isolated islets of B6 and 129 mice. Data shown are mean ± SEM. n = 3 for each strain. Two-way ANOVA with Bonferroni post hoc testing indicates: *, p < 0.05 vs. 3 mM glucose of the same strain; #, p < 0.05 vs. 129 with 16.7 mM glucose.
• Supplemental Table S1 - Primer sequences for real-time RT-PCR
• Supplemental Table S2 - Plasma insulin levels (ng/ml) in Ucp2+/+ and Ucp2-/- mice on different genetic backgrounds (Age= 3-6 mo)