Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplementary Table 1 - Predicted AREs in Sertoli cell expressed androgen-regulated genes.
• Supplemental Figure 1 - Characterization of Tg Abp.Cre mice. A, Map of linear transgene DNA construct encoding Cre recombinase with the rat androgen-binding protein gene (Abpa) promoter, used to produce Tg Abp.Cre founder mice. MT-1: metallothionein I sequence with polyA signal. B, Functional TgCre activity in Abp.Cre testes detected using ROSA-LacZ-reporter mice. β-galactoside staining shows the presence of Cre-loxP-mediated LacZ expression (blue color) in testes of Abp.Cre;ROSA mice (top), detected as early as 17 dpc. Fetal testis Cre expression in AMH.Cre mice was detected at 15 dpc (13). Age-matched non-Tg ROSA testes (bottom) provided negative controls. C, Localization of functional TgCre activity in testes from 9 week old Abp.Cre;ROSA or AMH.Cre;ROSA males. β-galactoside staining revealed equivalent patterns of Cre activity in the seminiferous tubules indicative of Sertoli cell-specific expression.